The presence of alpha-catenin in the VE-cadherin complex is required for efficient transendothelial migration of leukocytes.

International Journal of Biological Sciences
Jaap D van BuulPeter L Hordijk

Abstract

The majority of the leukocytes cross the endothelial lining of the vessels through cell-cell junctions. The junctional protein Vascular Endothelial (VE)-cadherin is transiently re-distributed from sites of cell-cell contacts during passage of leukocytes. VE-cadherin is part of a protein complex comprising p120-catenin and beta-catenin as intracellular partners. Beta-catenin connects VE-cadherin to alpha-catenin. This VE-cadherin-catenin complex is believed to dynamically control endothelial cell-cell junctions and to regulate the passage of leukocytes, although not much is known about the role of alpha- and beta-catenin during the process of transendothelial migration (TEM). In order to study the importance of the interaction between alpha- and beta-catenin in TEM, we used a cell-permeable version of the peptide encoding the binding site of alpha-catenin for beta-catenin (S27D). The data show that S27D interferes with the interaction between alpha- and beta-catenin and induces a reversible decrease in electrical resistance of the endothelial monolayer. In addition, S27D co-localized with beta-catenin at cell-cell junctions. Surprisingly, transmigration of neutrophils across endothelial monolayers was blocked in the presence of ...Continue Reading

References

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Jul 19, 2008·European Journal of Cell Biology·Magdalena J LorenowiczPeter L Hordijk

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Citations

Jun 23, 2011·PloS One·Giuseppina MarrazzoMichal Laniado Schwartzman
Dec 22, 2015·Brain, Behavior, and Immunity·Andreas WalkerHeidrun Potschka
May 13, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Elmar PieterseJohan van der Vlag

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Methods Mentioned

BETA
flow cytometry
Immunoprecipitation
FACS
pull-down
pull down
confocal microscopy

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