The presence of both the amino- and carboxyl-terminal domains in the AR is essential for the completion of a transcriptionally active form with coactivators and intranuclear compartmentalization common to the steroid hormone receptors: a three-dimensional imaging study

Molecular Endocrinology
Masayuki SaitohHajime Nawata

Abstract

To clarify the physiological significance of the intranuclear speckled distribution, or foci formation, of liganded steroid receptors, the subnuclear distribution of green (GFP), yellow (YFP), and cyan (CFP) fluorescent protein-tagged receptors and coactivators was investigated. The foci formation of 5 alpha-dihydrotestosterone (DHT)-bound AR-GFP in COS7 cells was abolished by the cotransfection of a CBP Delta (118-2393) fragment eliciting a dominant negative effect on the transactivation capacity of the AR. The N-terminal AR fragment (AR-AF-1-YFP), which has a strong constitutive transactivation function, formed foci without DHT, whereas the C-terminal AR fragment (AR-AF-2-CFP), which has a quite low transactivation function, was distributed homogeneously even in the presence of DHT. The reporter gene assay showed a synergism between the transactivation functions of AR-AF-1 and AR-AF-2. This synergism was not reflected by the above two-dimensional imaging. In contrast, a three-dimensional imaging method clearly showed a difference in the intranuclear spatial distribution. The DHT-bound wild-type AR-GFP alone or AR-AF-1-YFP plus DHT-bound AR-AF-2-CFP was distributed as approximately 300 discrete spots in one nucleus, whereas AR...Continue Reading

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Citations

Aug 18, 2006·Molecular and Cellular Biology·Yin WuRyoichi Takayanagi
Mar 4, 2003·Molecular and Cellular Biology·Marcel J M Schaaf, John A Cidlowski
Jul 19, 2013·Molecular Cancer Therapeutics·Sarah A LoddickA Nigel Brooks
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Jul 22, 2004·Annals of the New York Academy of Sciences·Nick Z Lu, John A Cidlowski
Jan 25, 2005·Molecular and Cellular Endocrinology·Hisaya KawateRyoichi Takayanagi
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May 11, 2005·Biochemical and Biophysical Research Communications·Masamichi IshizukaHiromi Hagiwara
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Jan 30, 2021·The Biochemical Journal·Martin StortzValeria Levi
Jan 18, 2006·Biochemical and Biophysical Research Communications·Shuli FanToshihiko Yanase

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