The presence of conjugative plasmid pLS20 affects global transcription of Its Bacillus subtilis host and confers beneficial stress resistance to cells

Applied and Environmental Microbiology
Thomas RöschP L Graumann

Abstract

Conjugation activity of plasmid pLS20 from Bacillus subtilis subsp. natto is induced when cells are diluted into fresh medium and diminishes as cells enter into stationary-phase growth. Transcriptional profiling shows that during mid-exponential growth, more than 5% of the host genes are affected in the presence of the plasmid, in contrast to the minor changes seen in freshly diluted and stationary-phase cells. Changes occurred in many metabolic pathways, although pLS20 does not confer any detectable burden on its host cell, as well as in membrane and cell wall-associated processes, in the large motility operon, and in several other cellular processes. In agreement with these changes, we found considerable alterations in motility and enzyme activity and increased resistance against several different forms of stress in cells containing the plasmid, revealing that the presence of pLS20 has a broad impact on the physiology of its host cell and increases its stress resistance in multiple aspects. Additionally, we found that the lack of chromosomal gene yueB, known to encode a phage receptor protein, which is upregulated in cells containing pLS20, strongly reduced conjugation efficiency, revealing that pLS20 not only increases fitne...Continue Reading

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Citations

May 7, 2014·PloS One·Laura A HuppertBriana M Burton
Sep 1, 2017·FEMS Microbiology Ecology·Xinlei JiangTerrence C K Lau
Aug 13, 2017·Applied and Environmental Microbiology·Dongfei HanWerner Liesack
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Apr 22, 2017·BMC Genomics·Christian KarlsenNils Peder Willassen
Aug 11, 2019·Microbiology Spectrum·Vincent J C van WindenMiriam Braunstein

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