PMID: 2491599Jan 1, 1989Paper

The presence of cryoprecipitable immunoglobulins in normal human sera may reflect specific molecular interactions

Autoimmunity
M A MaireP H Lambert

Abstract

The nature of the minute amounts of cryoprecipitable proteins present in all normal sera has been investigated in particular relation to the physicochemical and functional properties of the precipitated immunoglobulins. Cryoprecipitated material from 48 normal donor sera were first analyzed for their protein, IgG and IgM content. SDS-PAGE analysis revealed many bands in a majority of samples but in about one third a very limited number of bands was observed. IgM, IgG and albumin were identified and a lower molecular weight component (16 KD) was always present. Restricted clonality was demonstrated by IEF in 25-30% of the samples. In 50% of cryoprecipitates IgG was present in higher molecular weight fractions than 7S on sucrose density gradient at neutral pH. Ultracentrifugation at acid pH lead to a dissociation of these IgG fractions in most of the samples. A positive reaction for IgM rheumatoid factor was seen in 63% of the samples and there was a specific enrichment of RF activity in cryoprecipitates as compared to the corresponding serum. These data suggest that the cryoprecipitation of immunoglobulins in normal serum reflects specific interactions between immunoglobulin molecules rather than merely a non-specific precipitat...Continue Reading

Citations

Apr 1, 1997·Clinical Reviews in Allergy & Immunology·F Dammacco, D Sansonno
Nov 5, 2008·Journal of Clinical Immunology·Levon ManukyanGeorge Grigoryan
Oct 6, 1998·Journal of Immunological Methods·S Lacroix-DesmazesM D Kazatchkine
Jul 17, 2001·European Journal of Clinical Investigation·F DammaccoV Racanelli
Aug 27, 2011·Lancet·Manuel Ramos-CasalsXavier Bosch
Dec 21, 2007·Clinical Biochemistry·Anna BoyajyanRobert B Sim
Feb 10, 2007·Blood Reviews·Alessandra TedeschiEnrica Morra
Jan 24, 2007·Journal of Molecular Recognition : JMR·Vicky VallasPaul A Ramsland
Mar 19, 2016·World Journal of Clinical Cases·Belkiz OngenIbrahim Unsal
Dec 19, 2018·BioFactors·Umberto BasileGian Ludovico Rapaccini
Nov 20, 2019·Current Rheumatology Reports·Alejandro FuentesPaula I Burgos

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