The presence of F cells with a fetal phenotype in adults with hemoglobinopathies limits the utility of flow cytometry for quantitation of fetomaternal hemorrhage

Cytometry. Part B, Clinical Cytometry
Jad OthmanTeh-Liane Khoo

Abstract

Detection and quantitation of fetomaternal hemorrhage (FMH) can be difficult in patients with pre-existing elevations of HbF, such as those with hemoglobinopathies. The aim of this study was to evaluate the utility of dual-color flow cytometry with the Fetal Cell Count Kit (FCCK) in differentiating adult and fetal HbF in this population, as compared to flow cytometry (FC) using HbF alone. Peripheral blood was obtained from normal adults and patients with hemoglobinopathies (β-thalassemia and sickle cell disease), including a small number of pregnant females. Cord blood was used to spike some samples with 5% fetal cells. Analysis by single color (HbF) and dual-color (HbF and carbonic anhydrase) FC was performed on these samples. Fetal cells were defined as those with high HbF fluorescence on single-color FC, and those that were HbF + CA- using the FCCK. The quantity of fetal cells detected by each technique was compared. Forty-six adult patients were included. In non-pregnant adults with hemoglobinopathies, a population of red cells with a fetal cell phenotype were detected by both techniques. The dual-color method reported lower quantities of these cells. In nineteen samples spiked with cord blood the FCCK consistently underest...Continue Reading

References

Jul 28, 2006·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Mathie P G LeersVolkher Scharnhorst
Jun 3, 2008·Transfusion·Darcie J RadelDennis A Gastineau
Jun 29, 2012·Transfusion·Eugenia Prus, Eitan Fibach
Jun 21, 2016·International Journal of Laboratory Hematology·A GielezynskaE Kraszewska
Aug 6, 2016·Fetal and Pediatric Pathology·Mariela Granero FariasSergio H Almeida Martins Costa

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