The presence of masked antiribosomal P autoantibodies in healthy children

Arthritis and Rheumatism
C J AndersonH A Stafford

Abstract

To determine if antiribosomal P (anti-P) autoantibodies are present in healthy children. Sera from healthy children were screened for anti-P by conventional enzyme-linked immunosorbent assay and immunoblot techniques. Sera were also treated with immobilized ribosomal P antigens on nitrocellulose strips; affinity-purified fractions were tested for anti-P by high-sensitivity immunoblot. The relative binding affinities were compared for affinity-purified anti-P antibodies from healthy children and adults, and patients with systemic lupus erythematosus. IgG fractions of anti-P-depleted sera from healthy children were assessed for inhibition of autologous anti-P activity. Conventional serologic screening showed no IgG nor IgM anti-P in 88 untreated sera. IgG anti-P were unmasked in all 79 sera treated by the membrane batch affinity technique. IgM anti-P were identified in 27 of the treated sera; the percentage of positive sera decreased with increasing age (chi(2) for linear trend P = 0.00081). Affinity-purified anti-P from children had relative binding affinities similar to those of anti-P from other groups. Sera from healthy children contained inhibitory IgG antibodies to anti-P. These results show that anti-P autoantibodies are p...Continue Reading

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Citations

Sep 30, 1999·Current Opinion in Rheumatology·T Arkachaisri, T J Lehman
Mar 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shilpa OakChristiane S Hampe
Jan 11, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Wei Zhang, Morris Reichlin
Aug 22, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Z J PanH A Stafford
Feb 13, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·C J AndersonH A Stafford
Jul 3, 2004·Lupus·J T Merrill
Apr 16, 2013·Biochemistry. Biokhimii︠a︡·V N BunevaG A Nevinsky

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