PMID: 6160477Feb 11, 1980Paper

The presence of RNA in a double helix inhibits its interaction with histone protein

Nucleic Acids Research
K Dunn, J D Griffith

Abstract

The binding of core histones (H2A, H2B, H3, H4) to a circular plasmid DNA and to a circular DNA-RNA hybrid molecule of similar size has been compared. Circular hybrid molecules were formed from single stranded fd DNA by synthesis of the complimentary strand with ribonucleotides using wheat germ RNA polymerase II. Upon reconstitution of plasmid DNA circles with histone, the sedimentation profiles of the DNA remained sharp by increased several fold in rate. Material from the peak fractions of these sedimentations appeared to be condensed circular loops of nucleosomes when examined by electron microscopy (EM), and the mass ratio of DNA to histone (at the histone concentrations which produced the fastest sedimentations) was typical of native chromatin. In contrast, the sedimentation behavior of DNA-RNA hybrid circles after addition of histone remained unchanged except for a minor fraction which exhibited a broad and faster sedimentation rate. Examination by EM revealed that most of the molecules appeared identical to protein free hybrid circles while the minor, faster sedimenting fraction appeared to be two or more circles bound together by protein aggregates. Finally, a linear molecule consisting of about 3000 base pairs of duplex...Continue Reading

References

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Citations

Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Chrysogelos, J Griffith
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J NickolG Felsenfeld
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K R Hovatter, H G Martinson
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y H Wang, J D Griffith
Aug 1, 1985·Journal of Biomolecular Structure & Dynamics·D M Soumpasis
Jan 28, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mina NakamaYota Murakami
Apr 21, 2016·Nature Reviews. Molecular Cell Biology·Jessica S WilliamsThomas A Kunkel
May 6, 2014·DNA Repair·Jessica S Williams, Thomas A Kunkel
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Weston T PowellJanine M LaSalle
Jan 20, 1983·Biochimica Et Biophysica Acta·P E Prevelige, G D Fasman
Apr 14, 2017·Genome Research·Cornelia BrönnerMario Halic
Jan 1, 1982·CRC Critical Reviews in Biochemistry·I L CartwrightG C Howard
Sep 29, 1995·The Journal of Biological Chemistry·K P Nightingale, A P Wolffe
Oct 21, 2016·The Journal of Biological Chemistry·John M HinzJohn J Wyrick
Feb 29, 2020·The Journal of Biological Chemistry·Frederic Chedin, Craig J Benham
Jun 20, 2015·Frontiers in Genetics·Karen Usdin, Daman Kumari
Mar 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Robert StolzFrederic Chedin
Jan 20, 2021·Critical Reviews in Biochemistry and Molecular Biology·Zhi-Xiong ZhouThomas A Kunkel
Feb 9, 2021·Journal of Molecular Biology·Nathan Gamarra, Geeta J Narlikar
Jul 25, 2021·DNA Repair·Daisy Castillo-Guzman, Frédéric Chédin

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