PMID: 8944779Nov 1, 1996Paper

The presence of rotenone-sensitive NADH dehydrogenase in the long slender bloodstream and the procyclic forms of Trypanosoma brucei brucei

European Journal of Biochemistry
D S Beattie, M M Howton

Abstract

The mitochondrial electron-transport chain present in the procyclic and long slender bloodstream forms of Trypanosoma brucei brucei was investigated by means of several experimental approaches. The oxidation of proline, glycerol and glucose in procyclic cells was inhibited 80-90% by antimycin A or cyanide, 15-19% by salicylhydroxamic acid, and 30-35% by rotenone. Cytochrom-c-reductase activity, with proline or glycerol 3-phosphate as substrate, in a mitochondrial fraction isolated from these cells was inhibited by antimycin and rotenone, but not by malonate, while cytochrome-c-reductase activity with succinate as substrate was inhibited by antimycin A and malonate, but not by rotenone. In addition, the reduction of dichloroindophenol by NADH was inhibited by rotenone but not by malonate, which suggests that rotenone-sensitive NADH dehydrogenase (complex I) is present in these mitochondria. The presence of three subunits of NADH dehydrogenase was observed in immunoblots of mitochondrial proteins with specific antibodies raised against peptides corresponding to predicted antigenic regions of these proteins, which provides further evidence for the presence of NADH dehydrogenase. In long slender bloodstream forms, the oxidation of ...Continue Reading

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Citations

Jun 5, 2003·Archives of Biochemistry and Biophysics·Jing Fang, Diana S Beattie
Oct 12, 2001·International Journal for Parasitology·A Schneider
Jul 16, 2002·International Journal for Parasitology·Achim SchnauferKen Stuart
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Mar 5, 2003·Molecular and Biochemical Parasitology·Jing Fang, Diana S Beattie
Jun 26, 2014·Journal of Bioenergetics and Biomembranes·Margarida Duarte, Ana M Tomás
Nov 16, 2010·Molecular and Biochemical Parasitology·Zdeněk VernerJulius Lukes
Jul 4, 2002·The Journal of Biological Chemistry·Natacha Bochud-Allemann, André Schneider
Jan 8, 2021·Parasitology·Petra ČermákováAnton Horváth
Jun 19, 1998·Biochimica Et Biophysica Acta·A Videira

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