The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum

New Biotechnology
Nancy Coconi LinaresRonald P de Vries

Abstract

Guar gum consists mainly of galactomannan and constitutes the endosperm of guar seeds that acts as a reserve polysaccharide for germination. Due to its molecular structure and physical properties, this biopolymer has been considered as one of the most important and widely used gums in industry. However, for many of these applications this (hemi-)cellulosic structure needs to be modified or (partially) depolymerized in order to customize and improve its physicochemical properties. In this study, transcriptome, exoproteome and enzyme activity analyses were employed to decipher the complete enzymatic arsenal for guar gum depolymerization by Aspergillus niger. This multi-omic analysis revealed a set of 46 genes encoding carbohydrate-active enzymes (CAZymes) responding to the presence of guar gum, including CAZymes not only with preferred activity towards galactomannan, but also towards (arabino-)xylan, cellulose, starch and pectin, likely due to trace components in guar gum. This demonstrates that the purity of substrates has a strong effect on the resulting enzyme mixture produced by A. niger and probably by other fungi as well, which has significant implications for the commercial production of fungal enzyme cocktails.

References

Oct 6, 1998·Applied and Environmental Microbiology·N N van PeijL H de Graaff
Oct 22, 2002·FEBS Letters·Ronald P de VriesJaap Visser
Jul 9, 2004·Applied and Environmental Microbiology·Ronald P de VriesJaap Visser
Oct 16, 2012·Fungal Genetics and Biology : FG & B·Masahiro OgawaYasuji Koyama
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jane W AggerBjørge Westereng
Jun 4, 2014·Journal of Industrial Microbiology & Biotechnology·A R MalherbeW H van Zyl
Aug 4, 2015·Biotechnology for Biofuels·Isabelle BenoitRonald P de Vries
Nov 21, 2015·Ultrasonics Sonochemistry·Amrutlal L PrajapatParag R Gogate
Apr 6, 2016·International Journal of Biological Macromolecules·Nandkishore ThombareM Z Siddiqui
Jun 16, 2017·Biotechnology for Biofuels·Tiziano BenocciRonald P de Vries
Sep 28, 2017·Biotechnology for Biofuels·Areejit SamalN Louise Glass
Nov 25, 2017·BMC Genomics·Birgit S GrubenRonald P De Vries
Dec 12, 2017·Enzyme and Microbial Technology·Nicholas de Mojana di ColognaCarlos André Ornelas Ricart

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