PMID: 9542656May 23, 1998Paper

The presence of transcription factors in fetal bovine sera

In Vitro Cellular & Developmental Biology. Animal
P A KnepperE Hayes

Abstract

Three sources of fetal bovine serum (FBS) were fractionated by ammonium sulfate precipitation and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to Immobilon-P membranes, immunoblotted with a panel of transcription factor antibodies, and detected by enhanced chemiluminescence. Nine transcription factors were detected--ATF-2, SRE-ZBP, GATA-2, TFIID, Ets-1/Ets-2, E2F-1, Oct-2, p53, and AP-2; four transcription factors were not detected--Myo D, CREB, Sp2, and Wilms' tumor. The results indicated the presence of varying amounts of several transcription factors in three commercial sources and may represent heretofore unrecognized factors influencing cell culture.

References

Jan 1, 1992·Pharmacology & Therapeutics·U Bjare
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S Lai, W Herr
May 1, 1992·Molecular and Cellular Biology·R M Attar, M Z Gilman
Jun 6, 1991·Nature·A J LevineC A Finlay
Jan 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Prochiantz, L Théodore
Jun 30, 1995·The Journal of Biological Chemistry·N D LakinD S Latchman
Feb 24, 1995·The Journal of Biological Chemistry·M TowatariT Enver
Jan 1, 1994·Pharmacology & Therapeutics·A DornW Leupin
Oct 28, 1993·The New England Journal of Medicine·C C Harris, M Hollstein
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H E HuberD C Heimbrook
Feb 7, 1997·Cell·P J Follette, P H O'Farrell
Feb 27, 1997·Nature·I WilmutK H Campbell
Mar 21, 1997·Cell·W P Tansey, W Herr

❮ Previous
Next ❯

Citations

May 24, 2011·Stem Cells International·Chandana TekkatteKavitha Sankaranarayanan
Sep 7, 1999·In Vitro Cellular & Developmental Biology. Animal·P A KnepperD G McLone
Apr 25, 2002·Alternatives to Laboratory Animals : ATLA·Carlo E A JochemsVera Baumans

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.