PMID: 150289Aug 4, 1978

The presence of two (Na+ + K+)-ATPase inhibitors in equine muscle ATP: vanadate nad a dithioerythritol-dependent inhibitor

Biochimica Et Biophysica Acta
E E Quist, L E Hokin

Abstract

A potent inhibitor of (Na+ + K+)-ATPase activity was purified from Sigma equine muscle ATP by cation- and anion-exchange chromatography. The isolated inhibitor was identified by atomic absorption spectroscopy and proton resonance spectroscopy to be an inorganic vanadate. The isolated vanadate and a solution of V2O5 inhibit sarcolemma (Na+ + K+)-ATPase with an I50 of 1 micrometer in the presence of 1 mM ethyleneglycol-bis-(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA), 145 mM NaCl, 6mM MgCl2, 15 mM KCl and 2 mM synthetic ATP. The potency of the isolated vanadate is increased by free Mg2+. The inhibition is half maximally reversed by 250 micrometer epinephrine. Equine muscle ATP was also found to contain a second (Na+ + K+)-ATPase inhibitor which depends on the sulfhydryl-reducing agent dithioerythritol for inhibition. This unknown inhibitor does not depend on free Mg2+ and is half maximally reversed by 2 micrometer epinephrine. Prolonged storage or freeze-thawing of enzyme preparations decreases the susceptibility of the (Na+ + K+)-ATPase to this inhibitor. The adrenergic blocking agents, propranolol and phentolamine, do not block the catecholamine reactivation. The inhibitors in equine muscle ATP also inhibit highly purifi...Continue Reading

References

Jul 28, 1977·Nature·L A Beaugé, I M Glynn
Oct 18, 1977·Biochemistry·L Josephson, L C Cantley

Citations

Jun 18, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·G L NiederP A Culp
Jan 1, 1981·The Journal of Membrane Biology·L E Hokin
Oct 12, 2012·Archives of Environmental Contamination and Toxicology·Paula G SmithLoren Knopper
Jan 1, 1981·Pharmacology & Therapeutics·R S Kim, F S LaBella
Sep 5, 1981·Lancet
Aug 1, 1982·Psychological Medicine·D A DickE G Dick
Oct 4, 1979·Nature·T J Simons
Apr 1, 1982·Journal of Toxicology and Environmental Health·T D PhillipsA W Hayes
May 28, 1979·Biochemical and Biophysical Research Communications·G GruppA Schwartz
Nov 6, 1981·Biochimica Et Biophysica Acta·J P RossiA F Rega
Nov 1, 1994·Metabolism: Clinical and Experimental·S GanguliM Tucker
Aug 6, 1981·Biochimica Et Biophysica Acta·T RamasarmaF L Crane
Feb 11, 2015·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Prinessa Chellan, Peter J Sadler
Aug 10, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valter A NascimentoLourdes Z Z Consolo
Nov 1, 1984·Psychological Medicine·G J NaylorN I Ward
May 1, 1980·Basic Research in Cardiology·A HagenmeyerH Bader
May 1, 1980·Basic Research in Cardiology·H ScholzE Wetzel

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