The presequence of fumarase is exposed to the cytosol during import into mitochondria

Journal of Molecular Biology
S KarnielyO Pines

Abstract

The majority of mitochondrial proteins can be imported into mitochondria following termination of their translation in the cytosol. Import of fumarase and several other proteins into mitochondria does not appear to occur post-translationally according to standard in vivo and in vitro assays. However, the nature of interaction between the translation and translocation apparatuses during import of these proteins is unknown. Therefore, a major question is whether the nascent chains of these proteins are exposed to the cytosol during import into mitochondria. We asked directly if the presequence of fumarase can be cleaved by externally added mitochondrial processing peptidase (MPP) during import, using an in vitro translation-translocation coupled reaction. The presequence of fumarase was cleaved by externally added MPP during import, indicating a lack of, or a loose physical connection between, the translation and translocation of this protein. Exchanging the authentic presequence of fumarase for that of the more efficient Su9-ATPase presequence reduced the exposure of fumarase precursors to externally added MPP en route to mitochondria. Therefore, exposure to cytosolic MPP is dependent on the presequence and not on the mature par...Continue Reading

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Citations

Sep 2, 2009·The Journal of Biological Chemistry·Adi NaamatiOphry Pines
Mar 7, 2013·Molecular Biology and Evolution·Efrat BurakOphry Pines
May 23, 2007·Molekuliarnaia biologiia·P A KamenskiĭI A Tarasov
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
May 6, 2009·Molecular Microbiology·Neta Regev-RudzkiOphry Pines
Sep 21, 2011·The FEBS Journal·Ohad YogevOphry Pines
Sep 8, 2010·Developmental Disabilities Research Reviews·Shane L ReaMarni J Falk
Aug 26, 2009·Cell·Agnieszka ChacinskaNikolaus Pfanner
Mar 29, 2011·Journal of Molecular Biology·Reut Ben-MenachemOphry Pines
Mar 4, 2008·Biochimica Et Biophysica Acta·Nuno RaimundoAnu Suomalainen
Jul 11, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Neta Regev-Rudzki, Ophry Pines
Jun 9, 2006·American Journal of Physiology. Cell Physiology·Jean-Jacques BrièrePierre Rustin
Aug 2, 2007·The Journal of Biological Chemistry·Ohad YogevOphry Pines
Jan 1, 2014·Frontiers in Molecular Biosciences·Bella Kalderon, Ophry Pines
Mar 15, 2019·The Protein Journal·Katja G Hansen, Johannes M Herrmann
Nov 2, 2019·Mitochondrion·Carmela Vazquez-CalvoMartin Ott
Jun 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yardena SilasOphry Pines

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