The pressure effect on the structure and functions of protein disulfide isomerase

Biochimica Et Biophysica Acta
Kazuyoshi AdoY Taniguchi

Abstract

Studying on the pressure effects of the structure and functions of the multidomain protein, protein disulfide isomerase (PDI), the intrinsic Trp fluorescence spectra of PDI were measured under high pressure. PDI has 5 Trp residues and the two of all Trp residues are located at the neighborhood of the active site (WCGHC) for isomerase activity. On the basis of the red shift of center of spectral mass (CSM) of the intrinsic Trp fluorescence and the decrease in its fluorescence intensity, the changes in tertiary structure of PDI were observed above 100 MPa. These structural changes were completed at 400 MPa. The CSM of 400 MPa denatured PDI was comparable to that of 6.0 M GdnHCl denatured one. All of the Trp residues included in PDI are completely exposed to aqueous medium at 400 MPa. However, there is the significant difference between the pressure and GdnHCl-denatured PDI. The Trp fluorescence intensity was decreased with increasing pressure, but increased with the increase of the GdnHCl concentration. It is implied that the pressure-denatured state of PDI might remain compact not to be extensively unfolded. In the point of view about the reversibility of pressure-treated PDI, the tertiary structure was completely recovered afte...Continue Reading

References

Aug 14, 1987·Biochemical and Biophysical Research Communications·K YamauchiR Horiuchi
Dec 1, 1984·Biochemical Society Transactions·R B FreedmanN Lambert
Jul 15, 1995·European Journal of Biochemistry·J L Song, C C Wang
Dec 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C C Wang, C L Tsou
Feb 1, 1994·Protein Expression and Purification·R Noiva
Nov 5, 1997·The Journal of Biological Chemistry·Y Dai, C Wang
Sep 12, 1998·Biochimica Et Biophysica Acta·K Heremans, L Smeller
May 18, 1999·Journal of Molecular Biology·G DesaiC A Royer
Jun 26, 1999·Journal of Biomolecular NMR·J KemminkN J Darby
May 2, 2000·The Journal of Biological Chemistry·F ShaoJ C Bardwell
Oct 9, 2001·Trends in Biochemical Sciences·J L SilvaC A Royer
May 2, 2002·Biochimica Et Biophysica Acta·Catherine A Royer
Oct 18, 2002·Journal of Cellular Physiology·Carlo TuranoAnna Ferraro
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Sep 11, 2004·The Journal of Biological Chemistry·Rui TianRong-Qiao He

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Citations

Dec 29, 2010·Biochemistry Research International·Benjamin J PietersNorbert W Seidler
Sep 16, 2011·Biochemistry Research International·Michelle R BakerNorbert W Seidler
Jan 20, 2009·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Francisco Vargas-AlboresFlorinda Jiménez-Vega
Jun 19, 2007·Biochimica Et Biophysica Acta·Kazuyoshi Ado, Yoshihiro Taniguchi
Mar 15, 2008·The Journal of Gene Medicine·Gabriele CandianiMatteo Zanda
Feb 6, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hyong-Ha KimNam Woong Song

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