The Presumed Polyomavirus Viroporin VP4 of Simian Virus 40 or Human BK Polyomavirus Is Not Required for Viral Progeny Release

Journal of Virology
Stian HenriksenChristine Hanssen Rinaldo


The minor capsid protein of human BK polyomavirus (BKPyV), VP2, and its N-terminally truncated form, VP3, are both important for viral entry. The closely related simian virus 40 (SV40) reportedly produces an additional truncated form of VP2/3, denoted VP4, apparently functioning as a viroporin promoting progeny release. The VP4 open reading frame is conserved in some polyomaviruses, including BKPyV. In this study, we investigated the role of VP4 in BKPyV replication. By transfecting viral genomes into primary human renal proximal tubule epithelial cells, we demonstrated that unaltered BKPyV and mutants with start codon substitutions in VP4 (VP2M229I and VP2M229A) abolishing putative VP4 production were released at the same level to supernatants. However, during infection studies, VP2M229I and VP2M229A exhibited 90% and 65% reduced infectivity, respectively, indicating that isoleucine substitution inadvertently disrupted VP2/3 function to the detriment of viral entry, while inhibition of VP4 production during late infection was well tolerated. Unexpectedly, and similarly to BKPyV, wild-type SV40 and the corresponding VP4 start codon mutants (VP2M228I and VP2M228A) transfected into monkey kidney cell lines were also released at e...Continue Reading


Nov 4, 2017·Viruses·Francois HelleGilles Duverlie
Mar 20, 2020·MSphere·Robert Daniels, Daniel N Hebert
Aug 14, 2019·Frontiers in Oncology·John Charles RotondoFernanda Martini
Mar 20, 2020·MSphere·Stian Henriksen, Christine Hanssen Rinaldo


Aug 1, 1976·Virology·J AvilaG Khoury
Feb 13, 1992·Nature·J P GriffithD L Caspar
Nov 28, 1991·Nature·R C LiddingtonS C Harrison
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Feb 15, 1996·Structure·T StehleS C Harrison
Jun 17, 1998·Journal of Virology·C H RinaldoA Hey
Aug 11, 1998·Trends in Biochemical Sciences·R A BradshawK W Walker
Aug 20, 2002·The Journal of General Virology·Petra MannováJitka Forstová
Dec 20, 2002·Transplantation·Rui-Mei LiJeffrey B Kopp
Jun 19, 2003·The Journal of Biological Chemistry·Trond LamarkTerje Johansen
Oct 4, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Cinthia B DrachenbergEmilio Ramos
Jun 15, 2004·Virology·Jonathan LowMichael J Imperiale
Oct 17, 2006·Journal of Virology·M L GasparovicWalter J Atwood
Jul 31, 2007·PLoS Pathogens·Robert DanielsDaniel N Hebert
May 31, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Eva BernhoffChristine Hanssen Rinaldo
Sep 24, 2009·Journal of Medical Virology·Gunn-Hege OlsenChristine Hanssen Rinaldo
Mar 20, 2010·PLoS Pathogens·Tadaki SuzukiHirofumi Sawa
Jun 18, 2010·Transplant Infectious Disease : an Official Journal of the Transplantation Society·H OmarP Ljungman
Feb 12, 2011·Virus Research·Shoichiro TangeAkira Nakanishi
Feb 18, 2011·Journal of Clinical Microbiology·Alexis Dumoulin, Hans H Hirsch
Jan 13, 2012·Journal of Virology·Kristina M GiordaDaniel N Hebert
Mar 31, 2012·Reviews in Medical Virology·Marijke Van GhelueUgo Moens
Jul 4, 2012·Nature Reviews. Microbiology·José Luis NievaLuis Carrasco
Aug 4, 2012·PloS One·Rachel M SchowalterChristopher B Buck
Dec 12, 2012·The Journal of Biological Chemistry·Kristina M GiordaDaniel N Hebert
May 9, 2013·Biochemistry·Smita RaghavaDaniel N Hebert
Jun 7, 2013·Journal of Virology·Shauna M BennettMichael J Imperiale
Jun 21, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hans H HirschCeline Leboeuf
Jun 21, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Christine Hanssen RinaldoBiswa Nath Sharma
Apr 25, 2014·Journal of Virology·Stian HenriksenChristine Hanssen Rinaldo
Jun 17, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Bernhard Ehlers, Ugo Moens
Apr 1, 2015·The FEBS Journal·Liana Tsiatsiani, Albert J R Heck
Apr 22, 2015·Virology·Sara W Bird, Karla Kirkegaard

Related Concepts

Pathologic Cytolysis
Real-Time Polymerase Chain Reaction
VP3 protein, Rotavirus
VP4 protein, Rotavirus
Immunofluorescence Assay
Biochemical Pathway
Viral Release from Host Cell
Formicum acidum, formic acid, Homeopathic preparation

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.