Abstract
The prevalence of Clostridium botulinum types A, B, E and F in river lampreys caught in Finnish rivers was determined for the first time using a quantitative PCR-MPN (most probable number) analysis. One of 67 raw whole lampreys (1.5%) was positive for the botulinum neurotoxin type E gene, with the estimated C. botulinum count being 100spores/kg. Two type E strains were isolated from the positive sample and confirmed as different genotypes by pulsed-field gel electrophoresis. Although the current procedure of bringing the charcoal-broiled lampreys to market has been without any further packaging or extended storage, interest towards increasing the shelf life of the product by vacuum-packaging is increasing. Our results demonstrate that C. botulinum type E may constitute a safety hazard in processed lampreys from the Baltic Sea area if packaging and extended shelf lives are to be used. To control the potential risk, a storage temperature of 3 degrees C or below should be recommended for these products.
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