PMID: 7545961Dec 1, 1994Paper

The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodes a peptide inhibitor, iCF10

Journal of Bacteriology
J NakayamaA Suzuki

Abstract

Conjugative transfer of the Enterococcus faecalis tetracycline resistance plasmid pCF10 is stimulated by a peptide pheromone, cCF10. Once a recipient strain acquires pCF10 and thus becomes a pheromone-responsive donor, cCF10 activity is no longer detected in culture filtrates. Here we show that pCF10 encodes a peptide inhibitor, iCF10, secreted by donor cells; this inhibitor antagonizes the cCF10 activity in culture filtrates. In order to detect and quantitate iCF10, we developed a reverse-phase high-performance liquid chromatography assay in which the inhibitor peptide elutes separately from the pheromone; this type of assay enabled us to determine that lack of pheromone activity in donor culture filtrates was due to secretion of a mixture of iCF10 and cCF10, rather than abolition of cCF10 secretion. The gene encoding iCF10, prgQ, is located on the EcoRI-C fragment of pCF10. The open reading frame comprising the prgQ gene encodes a 23-amino-acid precursor that resembles a signal peptide. This precursor is cleaved to the mature heptapeptide iCF10 during the secretion process.

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Citations

May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anushree ChatterjeeWei-Shou Hu
Sep 9, 2011·PLoS Computational Biology·Che-Chi ShuDoraiswami Ramkrishna
Aug 17, 2011·Molecular Microbiology·Laura CookGary Dunny
Apr 28, 2017·Frontiers in Neuroscience·Frederick VerbekeBart De Spiegeleer
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ke ShiCathleen A Earhart
Oct 14, 2014·Frontiers in Cellular and Infection Microbiology·Juan Cristobal Jimenez, Michael J Federle
Sep 7, 2017·Annual Review of Genetics·Matthew B NeiditchMichael J Federle
Oct 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Josephine R ChandlerGary M Dunny
Oct 14, 2006·Molecular Microbiology·Briana K KozlowiczGary M Dunny
Oct 26, 2010·Molecular Microbiology·Lauren Mashburn-WarrenMichael J Federle
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gary M Dunny
Oct 28, 2016·Molecular Microbiology·Minny BhattyPeter J Christie
Mar 30, 2016·Journal of Bacteriology·Gary M Dunny, Ronnie Per-Arne Berntsson
Oct 15, 2013·FEMS Microbiology Reviews·Laura C Cook, Michael J Federle
May 22, 2016·Applied and Environmental Microbiology·Arpan BandyopadhyayWei-Shou Hu
Jun 14, 2019·Frontiers in Microbiology·Verena KohlerElisabeth Grohmann
Jul 23, 2013·Journal of Bacteriology·Breah LaSarreMichael J Federle
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anushree ChatterjeeWei-Shou Hu
Apr 3, 2020·PLoS Pathogens·Amy J SterlingJames S G Dooley
Oct 6, 2005·Applied and Environmental Microbiology·Christopher J KristichGary M Dunny
Mar 1, 1996·Trends in Microbiology·R WirthG Wanner
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Sep 18, 2004·Peptides·Josephine R Chandler, Gary M Dunny

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