The PRH/Hex repressor protein causes nuclear retention of Groucho/TLE co-repressors.

The Biochemical Journal
Cecile DesjobertP S Jayaraman

Abstract

The PRH (proline-rich homeodomain) [also known as Hex (haematopoietically expressed homeobox)] protein is a transcription factor that functions as an important regulator of vertebrate development and many other processes in the adult including haematopoiesis. The Groucho/TLE (transducin-like enhancer) family of co-repressor proteins also regulate development and modulate the activity of many DNA-binding transcription factors during a range of diverse cellular processes including haematopoiesis. We have shown previously that PRH is a repressor of transcription in haematopoietic cells and that an Eh-1 (Engrailed homology) motif present within the N-terminal transcription repression domain of PRH mediates binding to Groucho/TLE proteins and enables co-repression. In the present study we demonstrate that PRH regulates the nuclear retention of TLE proteins during cellular fractionation. We show that transcriptional repression and the nuclear retention of TLE proteins requires PRH to bind to both TLE and DNA. In addition, we characterize a trans-dominant-negative PRH protein that inhibits wild-type PRH activity by sequestering TLE proteins to specific subnuclear domains. These results demonstrate that transcriptional repression by PR...Continue Reading

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Citations

Nov 19, 2011·Leukemia·I HommingaJ P P Meijerink
Mar 28, 2009·Nucleic Acids Research·Abdenour SoufiPadma-Sheela Jayaraman
Aug 3, 2010·Nucleic Acids Research·Abdenour SoufiKevin Gaston
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Mar 5, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peter J NoyRoy Bicknell

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Methods Mentioned

BETA
PCR
protein assay
transfection
FCS
Assay
confocal microscopy
pulldown
co-immunoprecipitation

Software Mentioned

UNICORN
Leica Confocal
Molecular
ImageQuant
Dynamics

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