Mar 12, 1981

The primary charge separation, cytochrome oxidation and triplet formation in preparations from the green photosynthetic bacterium Prosthecochloris aestuarii

Biochimica Et Biophysica Acta
T SwarthoffJ Amesz


Flash-induced absorbance changes were measured in intact cells and subcellular preparations of the green photosynthetic bacterium Prosthecochloris aestuarii. In Complex I, a membrane vesicle preparation, photooxidation of the primary electron donor, P-840, and of cytochrome c-553 was observed. Flash excitation of the photosystem pigment complex caused in addition the generation of a bacteriochlorophyll a triplet. Triplet formation was the only reaction observed after flash excitation in the reaction center pigment-protein complex. The triplet had a lifetime of 90 microseconds at 295 K and of 165 microseconds at 120 K. The amount of triplet formed in a flash increased upon cooling from 295 to 120 K from 0.2 and 0.5 per reaction center to 0.45 and nearly 1 per reaction center in the photosystem pigment and reaction center pigment-protein complex, respectively. Measurements of absorbance changes in the near infrared in the reaction center pigment-protein complex indicate that the triplet is formed in the reaction center and that the reaction center bacteriochlorophyll a triplet is that of P-840. Formation of a carotenoid triplet did not occur in our preparations. Illumination with continuous light at 295 K of the reaction center p...Continue Reading

  • References10
  • Citations15


  • References10
  • Citations15


Mentioned in this Paper

Cytochrome c Oxidase Subunit VIa
Protein Complex Location
Triplet, Centriole
Nadh Dehydrogenase Complex

About this Paper

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