The primary structure of porcine aminoacylase 1 deduced from cDNA sequence

Journal of Biochemistry
M MittaS Tsunasawa


A cDNA encoding the complete amino acid sequence of aminoacylase 1 (N-acylamino acid aminohydrolase, ACY-1) [EC], a dimeric metalloprotein having two Zn2+ in the molecule, which catalyzes the deacylation of N-acylated L-amino acids except L-aspartic acid, has been isolated from porcine kidney lambda gt10 cDNA library and sequenced. From sequence analysis of the cDNA and the N- and C-terminal amino acid analyses of the purified protein, it is deduced that porcine kidney ACY-1 consists of two identical subunits (M(r) 45,260), each of which consists of a single chain of 406 amino acids with acetylalanine at the N-terminus. A cDNA encoding porcine liver ACY-1 was also cloned. The amino acid sequence deduced from the nucleotide sequence of the cDNA from porcine liver was identical to that deduced for porcine kidney ACY-1. Northern blot analysis suggested that ACY-1 is more highly expressed in kidney than in liver. Comparison of the amino acid sequence of porcine ACY-1 with those of other Zn2+-binding metalloenzymes showed no significant homologies in either the overall sequence or the consensus sequences for the metal binding sites. This indicates that ACY-1 is a new type of metalloprotein.


Sep 30, 2000·European Journal of Biochemistry·T GiardinaA Puigserver
Apr 19, 2003·Archives of Microbiology·Peter CurleyD van Sinderen
Mar 8, 2000·Bioscience, Biotechnology, and Biochemistry·M WakayamaM Moriguchi
Dec 1, 1996·International Journal of Peptide and Protein Research·Y YangH M Zhou
Sep 26, 2008·Applied Microbiology and Biotechnology·Rainer WardengaUwe Bornscheuer
Oct 3, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Debra NewmanAlexander Pushkin
Jan 17, 2020·Critical Reviews in Toxicology·Patrick E Hanna, M W Anders
Feb 17, 2021·JCI Insight·Debby NgoRobert E Gerszten
Jul 22, 1998·Chemical Research in Toxicology·V UttamsinghM W Anders
Oct 20, 1999·Chemical Research in Toxicology·V Uttamsingh, M W Anders
Mar 16, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A Biagini, A Puigserver
Dec 4, 2003·The International Journal of Biochemistry & Cell Biology·Qiang XieHai-Meng Zhou
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Qiang Xie, Hai-Meng Zhou
Jul 16, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Josette PerrierAntoine Puigserver
Jun 29, 2021·The Journal of General and Applied Microbiology·Junji HayashiMamoru Wakayama
Mar 31, 2005·Biochemical and Biophysical Research Communications·Sameh HergaThierry Giardina

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.