PMID: 8955311Dec 1, 1996Paper

The primase of broad-host-range plasmid R1162 is active in conjugal transfer

Journal of Bacteriology
D Henderson, R J Meyer

Abstract

The broad-host-range plasmid R1162 is conjugally mobilized at high frequency by the IncP-1 plasmid R751 but is poorly mobilized by pOX38, a derivative of the F factor. In both cases, the origin of transfer (oriT) and the Mob proteins of R1162 are required, indicating that these plasmids are mobilized by similar mechanisms. R1162 encodes a primase, essential for vegetative replication of the plasmid, that is made both as a separate protein and as the carboxy-terminal domain of MobA, one of the R1162 mobilization proteins (P. Scholz, V. Haring, B. Wittman-Liebold, K. Ashman, M. Bagdasarian, and E. Scherzinger, Gene 75:271-288, 1989). When R751 is the mobilizing vector, the primase is not required for mobilization of plasmids containing cloned mob-oriT R1162 DNA. However, detectable mobilization of such plasmids by pOX38 requires both the primase and its cognate initiation site, oriented for synthesis of the complement to the transferred strand. The long form of the primase is required for optimal transfer: R1162 replicons lacking this form also are not transferred detectably by pOX38 and are less well mobilized by R751. The distance between oriT and the primase initiation site affects the frequency of mobilization, and this effec...Continue Reading

References

Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·R B WeissR F Gesteland
Aug 1, 1986·Journal of Bacteriology·M A Brasch, R J Meyer
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·V HershfieldD R Helinski
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E M WongB Polisky
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·M S GuyerK B Low
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E ScherzingerM Bagdasarian
Jun 1, 1981·Genetical Research·N Willetts, C Crowther

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Citations

Oct 12, 2010·Biomolecular NMR Assignments·Nathan T WrightJoel F Schildbach
Feb 21, 2004·FEMS Microbiology Reviews·M Victoria FranciaFernando de la Cruz
Aug 27, 2016·Frontiers in Molecular Biosciences·Elisabeth GrohmannSabine Brantl
May 4, 2005·Journal of Bacteriology·Christopher Parker, Richard Meyer
Nov 1, 2011·Plasmid·Wesley Loftie-Eaton, Douglas E Rawlings
Dec 4, 2002·Plasmid·Christopher ParkerRichard Meyer
Mar 15, 2003·Biochimica Et Biophysica Acta·Lara M StreetJoel F Schildbach
Mar 2, 2005·Plasmid·Christopher ParkerRichard Meyer

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