The priming action of tumour necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on neutrophils activated by inflammatory microcrystals

Clinical and Experimental Immunology
H M Burt, J K Jackson

Abstract

We studied the effects of TNF-alpha or GM-CSF on the production of reactive oxygen species (as measured by chemiluminescence) and degranulation responses of neutrophils to opsonized inflammatory microcrystals. TNF-alpha in the 10-2000 pM or GM-CSF in the 2-200 pM concentration range caused the concentration-dependent amplification of neutrophil chemiluminescence responses to both calcium pyrophosphate dihydrate (CPPD) and monosodium urate monohydrate (MSUM) crystals. Degranulation responses, as measured by the extracellular release of the granule enzymes myeloperoxidase or lysozyme, were amplified by approximately 50-100% for both MSUM or CPPD crystal-induced neutrophil activation when cells were pretreated with TNF-alpha at 2000 pM or GM-CSF at 75 pM.

Citations

Nov 26, 2009·Immunology and Cell Biology·William John Martin, Jacquie L Harper
May 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lan PangBalázs Rada
Jul 29, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·J FäldtC Dahlgren
Dec 2, 2014·Case Reports in Rheumatology·Jácome Bruges-ArmasMatthew A Brown

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