The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores.

Purinergic Signalling
Florin TulucSatya P Kunapuli

Abstract

P2Y(2) receptors, which are equally responsive to ATP and UTP, can trigger intracellular signaling events, such as intracellular calcium mobilization and mitogen-activated protein (MAP) kinase phosphorylation in polymorphonuclear leukocytes (PMN). Moreover, extracellular nucleotides have been shown to prime chemoattractant-induced superoxide production. The aim of our study was to investigate the mechanism responsible for the priming effect of extracellular nucleotides on reactive oxygen species (ROS) production induced in human neutrophils by two different chemoattractants: formyl-methionyl-leucyl-phenylalanine (fMLP) and interleukin-8 (IL-8). Nucleotide-induced priming of ROS production was concentration- and time-dependent. When UTP was added to neutrophil suspensions prior to chemoattractant, the increase of the response reached the maximum at 1 min of pre-incubation with the nucleotide. UTP potentiated the phosphorylation of p44/42 and p38 MAP kinases induced by chemoattractants, however the P2 receptor-mediated potentiation of ROS production was still detectable in the presence of a SB203580 or U0126, supporting the view that MAP kinases do not play a major role in regulating the nucleotide-induced effect. In the presence...Continue Reading

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Citations

Nov 26, 2010·Purinergic Signalling·Guadalupe Martel-GallegosJorge Arreola
May 29, 2010·American Journal of Respiratory Cell and Molecular Biology·Monica LucattelliMarco Idzko
May 9, 2009·Trends in Parasitology·Rita Bhardwaj, Patrick J Skelly
Oct 30, 2014·Purinergic Signalling·Geoffrey Burnstock, Jean-Marie Boeynaems
Jun 4, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sanja CickoMarco Idzko
Jul 29, 2017·Frontiers in Pharmacology·Andromeda Liñán-RicoFievos L Christofi

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Methods Mentioned

BETA
electrophoresis

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