The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth.

Stem Cell Research & Therapy
Huan ZhouFa-Ming Chen

Abstract

Although dental pulp stem cells (DPSCs) isolated from periodontally compromised teeth (P-DPSCs) have been demonstrated to retain pluripotency and regenerative potential, their use as therapeutics remains largely unexplored. In this study, we investigated the proangiogenic effects of extracellular vesicles (EVs) secreted by P-DPSCs using in vitro and in vivo testing models. Patient-matched DPSCs derived from periodontally healthy teeth (H-DPSCs) were used as the control for P-DPSCs. Conditioned media (CMs) derived from H-DPSCs and P-DPSCs (H-CM and P-CM), CMs derived from both cell types pretreated with the EV secretion blocker GW4869 (H-GW and P-GW), and EVs secreted by H-DPSCs and P-DPSCs (H-EVs and P-EVs) were prepared to test their proangiogenic effects on endothelial cells (ECs). Cell proliferation, migration, and tube formation were assessed using the Cell Counting Kit-8 (CCK-8), transwell/scratch wound healing, and Matrigel assays, respectively. Specifically, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and western blot analysis were used to examine the expression levels of angiogenesis-related genes/proteins in ECs in response to EV-based incubation. Finally, a full-thickness skin defect model w...Continue Reading

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Citations

Jan 21, 2021·Regenerative Medicine·Antara BanerjeeSurajit Pathak
Jan 23, 2021·International Journal of Molecular Sciences·Oana BaruMîndra Badea
Apr 4, 2021·International Journal of Molecular Sciences·Jon LuzuriagaGaskon Ibarretxe
Nov 1, 2020·Archives of Oral Biology·Sijing YuBo Gao
May 4, 2021·Stem Cells International·Xiangyu HuangWenan Xu
Jul 3, 2021·International Journal of Molecular Sciences·Sarah Hani ShoushrahEdda Tobiasch
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Trinidad Montero-VilchezSalvador Arias-Santiago

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Methods Mentioned

BETA
flow cytometry
transmission electron microscopy
PCR
electrophoresis

Software Mentioned

Pro Plus
Image
ImageJ

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