The Probiotic Compound VSL#3 Modulates Mucosal, Peripheral, and Systemic Immunity Following Murine Broad-Spectrum Antibiotic Treatment

Frontiers in Cellular and Infection Microbiology
Ira EkmekciuMarkus M Heimesaat

Abstract

There is compelling evidence linking the commensal intestinal microbiota with host health and, in turn, antibiotic induced perturbations of microbiota composition with distinct pathologies. Despite the attractiveness of probiotic therapy as a tool to beneficially alter the intestinal microbiota, its immunological effects are still incompletely understood. The aim of the present study was to assess the efficacy of the probiotic formulation VSL#3 consisting of eight distinct bacterial species (includingStreptococcus thermophilus, Bifidobacterium breve, B. longum, B. infantis, Lactobacillus acidophilus, L. plantarum, L. paracasei, and L. delbrueckiisubsp.Bulgaricus) in reversing immunological effects of microbiota depletion as compared to reassociation with a complex murine microbiota. To address this, conventional mice were subjected to broad-spectrum antibiotic therapy for 8 weeks and perorally reassociated with either VSL#3 bacteria or a complex murine microbiota. VSL#3 recolonization resulted in restored CD4+ and CD8+ cell numbers in the small and large intestinal lamina propria as well as in B220+ cell numbers in the former, whereas probiotic intervention was not sufficient to reverse the antibiotic induced changes of respect...Continue Reading

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Citations

Mar 10, 2018·Probiotics and Antimicrobial Proteins·Renyuan GaoHuanlong Qin
Oct 17, 2019·Clinical Pharmacology and Therapeutics·Anukriti SharmaJack A Gilbert
Jan 19, 2019·Folia Microbiologica·Lucia HrncirovaTomas Hrncir
Aug 14, 2019·International Journal of Molecular Sciences·Ghalia KhoderSherif M Karam
Nov 7, 2019·Journal of Immunology Research·Li WangSong Qin
Apr 29, 2018·Scientific Reports·Eliane von KlitzingMarkus M Heimesaat
Nov 18, 2020·Critical Reviews in Food Science and Nutrition·Hui DuanWei Chen
Feb 26, 2019·Journal of Agricultural and Food Chemistry·Yizhe CuiChuang Xu

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Methods Mentioned

BETA
biopsies
PCR
flow cytometry
FACS

Software Mentioned

Immuco
Light Cycler Data Analysis
GraphPad Prism
FlowJo

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