PMID: 9445000Jan 28, 1998Paper

The product of the herpes simplex virus type 1 UL25 gene is required for encapsidation but not for cleavage of replicated viral DNA

Journal of Virology
A R McNabF L Homa

Abstract

The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison et al., Virology 138:246-259, 1984). In this study, we describe the isolation of a UL25 mutant (KUL25NS) that was constructed by insertion of an in-frame stop codon in the UL25 open reading frame and propagated on a complementing cell line. Although the mutant was capable of synthesis of viral DNA, it did not form plaques or produce infectious virus in noncomplementing cells. Antibodies specific for the UL25 protein were used to demonstrate that KUL25NS-infected Vero cells did not express the UL25 protein. Western immunoblotting showed that the UL25 protein was associated with purified, wild-type HSV A, B, and C capsids. Transmission electron microscopy indicated that the nucleus of Vero cells infected with KUL25NS contained large numbers of both A and B capsids but no C capsids. Analysis of infected cells by sucrose gradient sedimentation analysis confirmed that the ratio of A to B capsids was elevated in KUL25NS-i...Continue Reading

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Citations

Dec 10, 1999·Journal of Virology·R J RollerD DeSalvo
Aug 15, 2003·The Journal of General Virology·Nathalie Desloges, Claire Simard
Feb 14, 2006·Journal of Virology·Brian R BowmanFlorante A Quiocho
Dec 15, 2010·Protein & Cell·Haitao GuoHongyu Deng
Apr 16, 2011·Nature Reviews. Microbiology·David C Johnson, Joel D Baines
Sep 2, 2011·Journal of Virology·Mindy LeelawongGregory A Smith
Sep 27, 2015·Proceedings of the National Academy of Sciences of the United States of America·Nicholas J HuffmasterGregory A Smith
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D G WolfE S Mocarski
Jan 18, 2006·Journal of Virology·Walt E AdamsonFrazer J Rixon
Jun 14, 2003·Journal of Virology·Susan L BjerkeRichard J Roller
Jun 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wouter H RoosGijs J L Wuite
Aug 24, 2007·Journal of Virology·Kelly Elizabeth CollerGregory Allan Smith
Jun 26, 2009·Journal of Virology·Frauke BeilsteinNigel D Stow
Oct 24, 2008·Journal of Virology·Shelley K CockrellFred L Homa
Jul 7, 2017·Journal of Virology·Ryosuke KobayashiYasushi Kawaguchi

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