PMID: 9420254Jan 7, 1998Paper

The product of the respiratory syncytial virus M2 gene ORF1 enhances readthrough of intergenic junctions during viral transcription

Journal of Virology
R Hardy, G W Wertz

Abstract

The mRNA encoding the M2 protein of respiratory syncytial (RS) virus contains two open reading frames (ORFs). ORF1 encodes the 22-kDa structural protein, M2, and ORF2 has the potential to encode a 10-kDa protein (90 amino acids). Using a vaccinia virus T7 expression system, we examined the RNA synthetic activities of mono- and dicistronic subgenomic replicons of RS virus by direct metabolic labeling of RNA in the presence and absence of the products of ORF1 and ORF2. In the absence of ORF1 and ORF2, the negative- and positive-sense products of genomic RNA replication and positive-sense polyadenylated mRNA(s) were synthesized. Expression of the whole M2 transcription unit (containing ORF1 and ORF2) or ORF1 alone caused an increase in the synthesis of polyadenylated mRNA, the majority of which was due to a substantial increase in the quantity of polycistronic mRNAs generated by the polymerase failing to terminate at gene end signals. In agreement with previous reports, the ORF2 product was found to inhibit viral RNA replication and mRNA transcription. These data show that the M2 protein functions as a transcriptional antiterminator that enhances the ability of the viral RNA polymerase to read through intergenic junctions. The rol...Continue Reading

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Citations

Jan 19, 2002·Journal of Virology·Rachel FearnsPeter L Collins
Jun 8, 2002·The Journal of Biological Chemistry·Jens ModrofStephan Becker
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Jul 16, 2004·Journal of Virology·Sander HerfstRon A M Fouchier
Dec 28, 2005·Journal of Virology·Sven EnterleinElke Mühlberger
Nov 15, 2008·The Journal of General Virology·Miranda de GraafEdward C Holmes
Aug 31, 1999·The Journal of General Virology·G AhmadianA J Easton
Jan 30, 2007·Veterinary Research·Jean-Francois Valarcher, Geraldine Taylor
Jul 22, 2010·Molecular & Cellular Proteomics : MCP·Diane C MundayJulian A Hiscox
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sian J TannerJohn N Barr
Jun 13, 2015·Clinical and Vaccine Immunology : CVI·Sherry C Wen, John V Williams
Apr 6, 2016·Expert Opinion on Drug Discovery·Robert Cox, Richard K Plemper
May 2, 2007·Annals of the New York Academy of Sciences·Shobha Broor, Preeti Bharaj
Apr 16, 2004·Clinical Microbiology Reviews·Andrew J EastonHelene F Rosenberg

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