The production of anti-hexapeptide antibodies which recognize the S7, L6 and L13 ribosomal proteins of Escherichia coli

Journal of Peptide Science : an Official Publication of the European Peptide Society
Aristomenis MetaxasMaria Liakopoulou-Kyriakide

Abstract

Here we report the synthesis of the N-terminal hexapeptide H-Pro-Arg-Arg-Arg-Val-Ile-OH of the E. coli ribosomal protein S7. the C-terminal hexapeptide H-Lys-Glu-Ala-Lys-Lys-Lys-OH of L6 and the C-terminal hexapeptide H-Pro-Gln-Val-Leu-Asp-Ile-OH of L13. All peptides were prepared by SPPS following the Fmoc-strategy, using DIC/HOBt and/or HBTU as coupling reagents and 2-chlorotrityl chloride resin as the solid support. The carrier linked synthetic peptides were injected into rabbits and elicited an anti-peptide response. These anti-hexapeptide antibodies were found to recognize the corresponding peptides and proteins.

References

Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Feb 11, 1983·Science·J G SutcliffeR A Lerner
Apr 1, 1983·Molecular Immunology·T P Hopp, K R Woods

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Citations

Oct 1, 2007·Chemical Physics Letters·Leo SeballosJin Z Zhang

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