The production of multi-transgenic pigs: update and perspectives for xenotransplantation

Transgenic Research
H Niemann, Bjoern Petersen

Abstract

The domestic pig shares many genetic, anatomical and physiological similarities to humans and is thus considered to be a suitable organ donor for xenotransplantation. However, prior to clinical application of porcine xenografts, three major hurdles have to be overcome: (1) various immunological rejection responses, (2) physiological incompatibilities between the porcine organ and the human recipient and (3) the risk of transmitting zoonotic pathogens from pig to humans. With the introduction of genetically engineered pigs expressing high levels of human complement regulatory proteins or lacking expression of α-Gal epitopes, the HAR can be consistently overcome. However, none of the transgenic porcine organs available to date was fully protected against the binding of anti-non-Gal xenoreactive natural antibodies. The present view is that long-term survival of xenografts after transplantation into primates requires additional modifications of the porcine genome and a specifically tailored immunosuppression regimen compliant with current clinical standards. This requires the production and characterization of multi-transgenic pigs to control HAR, AVR and DXR. The recent emergence of new sophisticated molecular tools such as Zinc-F...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Osterud, S I Rapaport
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B M MuellerW Ruf
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·H Y SongD V Goeddel
Mar 21, 1998·The Journal of Clinical Investigation·G C ParryN Mackman
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·H H ChouA Varki
Mar 5, 2002·Nature Biotechnology·Yifan DaiDavid L Ayares
Dec 21, 2002·Science·Carol J PhelpsDavid L Ayares
Feb 5, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hatem ZayedZoltán Ivics
Feb 15, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Christian P LarsenRobert J Peach
Feb 25, 2006·Xenotransplantation·Eduardo DavilaChristopher G A McGregor
Nov 15, 2006·Transplantation·Sandra CrikisAnthony J F d'Apice
Jun 16, 2007·Nature Reviews. Immunology·Yong-Guang Yang, Megan Sykes
Feb 19, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Bjoern PetersenHeiner Niemann
May 1, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J C RousselP J Cowan
Jul 10, 2008·Xenotransplantation·K F Eddy LeePeter J Cowan
Sep 25, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jens LutzPaul C Evans
Oct 30, 2008·Xenotransplantation·Guerard W ByrneChristopher G A McGregor
Nov 1, 2008·Transplant Immunology·Anthony J F d'Apice, Peter J Cowan
Jul 21, 2009·Methods : a Companion to Methods in Enzymology·Zsuzsanna IzsvákZoltán Ivics
Jan 1, 2010·Xenotransplantation·Björn PetersenHeiner Niemann
Aug 19, 2010·Nature Reviews. Genetics·Fyodor D UrnovPhilip D Gregory
Dec 2, 2010·Transplantation·Guerard W ByrneChristopher G A McGregor
Dec 24, 2010·Nature Biotechnology·Jeffrey C MillerEdward J Rebar
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Janet HauschildHeiner Niemann
Jan 25, 2012·Journal of Molecular and Cellular Cardiology·Debra G WheelerRichard J Gumina

❮ Previous
Next ❯

Citations

Feb 7, 2019·In Vitro Cellular & Developmental Biology. Animal·Min Seong KimSeung Tae Lee
Mar 29, 2019·Transplant International : Official Journal of the European Society for Organ Transplantation·Franka MessnerGerald Brandacher
Sep 7, 2017·Virology Journal·Joachim Denner
Jun 21, 2019·Xenotransplantation·Kannan P SamyAllan D Kirk
Nov 5, 2019·Science Immunology·Megan Sykes, David H Sachs
Sep 25, 2017·Science·Joachim Denner
Sep 1, 2016·Pleura and Peritoneum·Kunio Kawanishi
Jan 1, 2016·Reproduction, Fertility, and Development·Santiago JosaLluis Montoliu
Apr 18, 2018·Tissue Engineering. Part a·Siddharth PatelLuiz E Bertassoni
Nov 18, 2018·EMBO Molecular Medicine·Hongyong ZhangQi Zhou
Jul 22, 2016·Xenotransplantation·Vladimir A MorozovJoachim Denner
Jun 8, 2017·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Almudena FernándezLluis Montoliu
Apr 10, 2019·Xenotransplantation·Laura Martínez-AlarcónPascual Parrilla
Apr 23, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Hai-Chang YinQing-Wen Meng
May 2, 2020·Cell and Tissue Research·Reinhard Pabst
Jul 7, 2019·Metabolites·Daniel SchultzMichael Lalk
Apr 24, 2016·Xenotransplantation·Christopher Burlak
Apr 5, 2016·Oral Diseases·A Peterson
Jul 19, 2017·Xenotransplantation·Kefeng YangOuliana Ziouzenkova
Nov 3, 2020·Frontiers in Genetics·Luiz Sergio Almeida CamargoPablo Juan Ross
Dec 30, 2020·Scandinavian Journal of Immunology·Sabin J BozsoJeevan Nagendran
Jun 29, 2021·Zygote : the Biology of Gametes and Early Embryos·Jongki ChoChung-Gyu Park
Jun 20, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jinfu ZhangShengsong Xie
Jul 8, 2021·Transgenic Research·Joohyun ShimKimyung Choi
Jul 3, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Ronald F ParsonsUNKNOWN ASTS Cellular Transplantation Committee
Jul 9, 2021·Nature Biotechnology·Koko KwisdaNils Hoppe
Sep 14, 2017·Current Opinion in Organ Transplantation·Burcin EkserDavid K C Cooper

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Related Papers

Molecular Reproduction and Development
Nikolai KlymiukEckhard Wolf
Reproduction, Fertility, and Development
A J FrenchP S Mountford
Cellular and Molecular Life Sciences : CMLS
J L BracyJ Iacomini
Kidney International
B SprangersA D Billiau
Clinical Nephrology
Emanuele CozziD J White
© 2021 Meta ULC. All rights reserved