The production of transforming growth factor-beta activity by rat granulosa cell cultures

Endocrinology
I C Kim, D W Schomberg

Abstract

We have examined whether granulosa cells (GC) secrete transforming growth factor-beta (TGF beta)-like activity using cell cultures prepared from diethylstilbestrol-primed female rats. Our results indicate that a significant level of active as well as latent TGF beta activity is found in defined GC culture medium as assessed by 1) potentiation of FSH-induced differentiation of rat GC, 2) neutralization of its activity by anti-TGF beta immunoglobulin, 3) inhibition of DNA synthesis in mink lung epithelial cells (CCl 64), and 4) activation of latent TGF beta activity by either acid or heat treatment. TGF beta production was more pronounced when the cells were seeded on fibronectin-coated plates. There was no difference in the level of TGF beta secretion by GC preparations derived from either diethylstilbestrol-primed immature or normal immature rats or adult rats. Furthermore, rat GC-conditioned medium contained much more TGF beta activity than medium from normal rat kidney cells (NRK 49-F), human prostatic adenocarcinoma cells (PC-3), or porcine GC. Rat thecal/interstitial cell culture medium contained activity comparable to that of GC medium. We conclude that rat GC preparations secrete a high level of TGF beta activity in vitro...Continue Reading

Citations

Jan 1, 1991·Molecular Reproduction and Development·C BruckerB A Sandow
Oct 1, 1994·Molecular Reproduction and Development·M M Shull, T Doetschman
May 1, 1992·Molecular and Cellular Endocrinology·H EngelhardtD T Armstrong
Jun 1, 1993·Molecular and Cellular Endocrinology·B K GangradeJ V May
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·O AvalletJ M Saez
Oct 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·E Y Adashi
Jun 24, 2003·Animal Reproduction Science·Philip G Knight, Claire Glister
Sep 15, 2007·Reproductive Biomedicine Online·Roberto Palermo
Jul 9, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Teddy ChanEdward E Tredget
Feb 8, 2008·Reproduction, Fertility, and Development·Thomas PapanikolaouConstantinos A Rekkas
Oct 8, 2009·The Journal of Reproduction and Development·Qiang WengKazuyoshi Taya
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Phil G Knight, Claire Glister
Jan 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D R Rowley
Oct 28, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Camilla AlexandersonAgneta Holmäng
Oct 1, 1996·Differentiation; Research in Biological Diversity·C LevacherR Habert
May 22, 2002·Biology of Reproduction·Patricia E SaragüetaJ Lino Barañao
May 28, 1993·Annals of the New York Academy of Sciences·M BenahmedP Franchimont
Feb 1, 1996·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·F Scheele, J Schoemaker
Jul 1, 2020·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Seda KarabulutIlknur Keskin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.