The production, purification and crystallization of a soluble form of the nonclassical MHC HLA-G: the essential role of cobalt

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Craig S ClementsJamie Rossjohn

Abstract

HLA-G is a nonclassical class I major histocompatibility complex (MHC) molecule that is primarily expressed at the foetal-maternal interface. Although the role of HLA-G has not been fully elucidated, current evidence suggests it protects the foetus from the maternal immune response. In this report, HLA-G (44 kDa) is characterized by expression in Escherichia coli. The inclusion bodies were refolded in complex with a peptide derived from histone H2A (RIIPRHLQL), purified and subsequently crystallized. Correct refolding was determined using two conformation-dependent antibodies. Cobalt ions were shown to be an essential ingredient for obtaining diffraction-quality crystals. The crystals, which diffracted to 1.9 A resolution, belonged to space group P3(2)2(1), with unit-cell parameters a = b = 77.15, c = 151.72 A.

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Citations

May 15, 2013·Nature Immunology·Richard BerryJamie Rossjohn
Apr 3, 2007·Human Immunology·Craig S ClementsJamie Rossjohn
Feb 4, 2010·Journal of Molecular Biology·Nicholas G WalpoleCraig S Clements
Sep 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roberta RizzoFrancesco Di Virgilio
Mar 12, 2015·The Journal of Biological Chemistry·Shoeib MoradiJulian P Vivian

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