The profiles of miRNAs and lncRNAs in peripheral blood neutrophils exosomes of diffuse cutaneous systemic sclerosis.

Journal of Dermatological Science
Liya LiHonglin Zhu

Abstract

Diffuse cutaneous systemic sclerosis (dSSc) is a systemic autoimmune disease with skin fibrosis. Neutrophils display important roles in autoimmunity, inflammation, vasculopathy and fibrosis. Exosomes (EXOs) are cell-derived vesicles contained various noncoding RNAs, mRNA and proteins with biological roles. To investigate the roles of miRNAs and lncRNAs from dSSc neutrophils EXOs. EXOs were isolated from cultured neutrophils supernatants and identified by transmission electron microscopy. Global expression of miRNAs and lncRNAs in neutrophils EXOs were sequenced by Illumina HiSeq 3000 and bioinformatic analyses were performed by R/Bioconductor. Genes were validated by real-time quantitative PCR. In profiles of neutrophils EXOs, we identified 22 dysregulated miRNAs and 281 dysregulated lncRNAs. Predicted target genes of them were enriched in GO, KEGG and Reactome pathways, Wnt, AMPK, IL-23 and NOTCH signaling pathways were selected for further analysis. Widely interactions among them were also found. Human dermal microvascular endothelial cells and human primary skin fibroblasts were stimulated with dSSc neutrophils EXOs, these fibrosis related genes were detected and some changes were found, such as ENST00000533886.1-hsa-miR-126...Continue Reading

Citations

Feb 15, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mingchen XiongYiping Wu
May 11, 2021·Journal of Biomedical Science·Angelo Giuseppe CondorelliDaniele Castiglia
May 20, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chenggui MiaoJinling Huang
Oct 31, 2021·Immunologic Research·Yogesh SharmaReena V Saini

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