The profiles of mitochondrial respiration and glycolysis using extracellular flux analysis in porcine enterocyte IPEC-J2

Animal Nutrition
Bie TanYulong Yin

Abstract

The porcine intestinal mucosa require large amounts of energy for nutrient processing and cellular functions and is vulnerable to injury by weaning stress involving bioenergetics failure. The mitochondrial bioenergetic measurement in porcine enterocytes have not been defined. The present study was to establish a method to measure mitochondrial respiratory function and profile mitochondrial function of IPEC-J2 using cell mito stress test and glycolysis stress test assay by XF24 extracellular flux analyzer. The optimal seeding density and concentrations of the injection compounds were determined to be 40,000 cells/well as well as 0.5 µM oligomycin, 1 µM carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (FCCP) and 1 µM rotenone & antimycin A, respectively. The profiles of mitochondrial respiration and glycolysis confirmed that porcine enterocyte preferentially derived much more energy from glutamine than glucose. These results will provide a basis for further study of mitochondrial function and bioenergetics of the porcine small intestine.

Citations

Oct 6, 2018·American Journal of Medical Genetics. Part a·Merlin G ButlerPartha C Krishnamurthy
Sep 25, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Elizabeth S SilagiMakarand V Risbud
Feb 15, 2019·Frontiers in Immunology·Marita ChakhtouraStefania Gallucci
Nov 24, 2020·The International Journal of Biochemistry & Cell Biology·Suryakant NitureDeepak Kumar
Jun 3, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Mohammad Golam SabbirPeter Zahradka
Apr 29, 2019·Psychoneuroendocrinology·Caroline TrumpffMartin Picard
Jun 11, 2021·Frontiers in Molecular Biosciences·Chiara BernardiniSalvatore Nesci

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