The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails

European Journal of Cell Biology
Yu LiRoger Karlsson

Abstract

A unique set of affinity-purified anti-profilin and anti-actin antibodies generated against a covalently coupled version of the profilin:actin complex was used to assess the distribution of profilin and non-filamentous actin in mouse melanoma cells. In agreement with the profilin:actin complex being the principal source of actin for filament formation, we observed extensive co-distribution of both antibody preparations with vasodilator-stimulated phosphoprotein (VASP) and the p34 subunit of the Arp2/3 complex, both of which are components of actin polymer-forming protein complexes in the cell. This suggests that the localization of profilin and actin revealed with these antibodies in fact reflects the distribution of the profilin:actin complex rather than the two proteins separately. Significantly, protruding lamellipodia and filopodia showed intensive labeling. The two antibody preparations were also used to stain HeLa cells infected with Listeria monocytogenes or vaccinia virus. In both cases, the pattern of antibody staining of the pathogen-induced microfilament arrangement differed, suggesting a varying accessibility for the antibody-binding epitopes.

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Citations

Apr 22, 2010·Genetic Testing and Molecular Biomarkers·Ziad KanaanSusan Galandiuk
Oct 19, 2012·Cell Adhesion & Migration·Zhijie DingPartha Roy
Jun 17, 2016·Molecular Biology of the Cell·Michaela NejedlaRoger Karlsson
May 14, 2011·Cellular and Molecular Bioengineering·Dave GauPartha Roy
Dec 8, 2019·Cytoskeleton·Rita Pinto-Costa, Mónica M Sousa
Oct 14, 2020·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Yuwei LingZhenwen Chen

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