The prognostic efficacy of cell-free DNA hypermethylation in colorectal cancer

Oncotarget
Simon Ladefoged RasmussenOle Thorlacius-Ussing

Abstract

Epigenetic alterations in colorectal cancer (CRC) cause important differences in the underlying tumor biology and aggressiveness. DNA hypermethylation is central for the development of CRC but the prognostic impact remains elusive. We aimed to assess the association between cell-free hypermethylated DNA and stage and survival in colorectal cancer (CRC). We analyzed pre-treatment plasma samples from 193 patients with CRC. Thirty gene-promoter regions were analyzed using methylation specific PCR. We compared the median number (range) of hypermethylated promoter regions with CRC stage, and constructed a multivariable Cox-regression model adjusted for stage, to evaluate the added prognostic information. The median number of hypermethylated promoter regions was nine (0-28) in patients with distant metastasis compared to five (0-19) in patients without metastatic disease (p < 0.0001). The majority of the hypermethylated promoter regions inferred a poor prognosis. Cox-regression analysis adjusted for patient age, sex, pre-treatment CEA-levels, and disease stage, showed thatRARB(HR = 1.99, 95% CI [1.07, 3.72]) andRASSF1A(HR = 3.35, 95% CI [1.76, 6.38]) hypermethylation inferred a significant effect on survival. The risk of metastasis i...Continue Reading

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Citations

Aug 9, 2018·Frontiers in Oncology·Hariti SalujaErin L Symonds
Apr 5, 2019·Disease Markers·Zhiyao MaWai K Leung
Aug 16, 2019·Archives of Biochemistry and Biophysics·Jennifer L MeitzlerJames H Doroshow
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Aitor Rodriguez-CasanovaAngel Diaz-Lagares
Jul 3, 2021·Cancers·Cora Palanca-BallesterAlfonso Calvo

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Methods Mentioned

BETA
PCR
biopsy
blood drawn
deamination

Clinical Trials Mentioned

NCT02928120

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