The prognostic value of JUNB-positive CTCs in metastatic breast cancer: from bioinformatics to phenotypic characterization

Breast Cancer Research : BCR
Galatea KallergiVassilis Georgoulias

Abstract

Circulating tumor cells (CTCs) are important for metastatic dissemination of cancer. They can provide useful information, regarding biological features and tumor heterogeneity; however, their detection and characterization are difficult due to their limited number in the bloodstream and their mesenchymal characteristics. Therefore, new biomarkers are needed to address these questions. Bioinformatics functional enrichment analysis revealed a subgroup of 24 genes, potentially overexpressed in CTCs. Among these genes, the chemokine receptor CXCR4 plays a central role. After prioritization according to the CXCR4 corresponding pathways, five molecules (JUNB, YWHAB, TYROBP, NFYA, and PRDX1) were selected for further analysis in biological samples. The SKBR3, MDA-MB231, and MCF7 cell lines, as well as PBMCs from normal (n = 10) blood donors, were used as controls to define the expression pattern of all the examined molecules. Consequently, 100 previously untreated metastatic breast cancer (mBC) patients (n = 100) were analyzed using the following combinations of antibodies: CK (cytokeratin)/CXCR4/JUNB, CK/NFYA/ΥWHΑΒ (14-3-3), and CK/TYROBP/PRDX1. A threshold value for every molecule was considered the mean expression in normal PBMCs. ...Continue Reading

References

May 1, 1991·Molecular and Cellular Biology·Y DevaryM Karin
Sep 1, 1991·Molecular and Cellular Biology·K Kovary, R Bravo
Dec 10, 1998·Nucleic Acids Research·H OgataM Kanehisa
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Oct 30, 2001·Behavioural Brain Research·Y BenjaminiI Golani
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Aug 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A StathopoulouV Georgoulias
Jul 2, 2004·Nature Reviews. Cancer·Fran Balkwill
Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Oct 9, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christopher J LarsonLeon W M M Terstappen
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Songdong MengJonathan W Uhr
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Jun 14, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nikos XenidisDimitris Mavroudis
Apr 12, 2008·Nature Reviews. Cancer·Klaus PantelBurkhard Brandt
Jul 22, 2009·Cancer Letters·Panayiotis A TheodoropoulosVassilis Georgoulias
Feb 27, 2010·Science·Jocelyn Kaiser
Jun 8, 2010·BMC Systems Biology·Ettore MoscaLuciano Milanesi
Jan 26, 2011·PloS One·Michail IgnatiadisChristos Sotiriou
Mar 16, 2013·The Breast : Official Journal of the European Society of Mastology·Petra SeiboldJenny Chang-Claude
Jun 20, 2013·Molecular Cancer Therapeutics·Galatea KallergiSofia Agelaki
Dec 18, 2013·Genetics and Molecular Research : GMR·Y GongB Wu
May 3, 2014·International Journal of Oncology·C McDonaldS A Phelan
May 9, 2014·IEEE Journal of Biomedical and Health Informatics·Stelios SfakianakisDimitrios Kafetzopoulos
May 27, 2014·Biochemical and Biophysical Research Communications·Mellissa HicksJames DeWille
Jun 2, 2014·Nature Medicine·Cassandra L HodgkinsonCaroline Dive
Aug 20, 2015·Breast Cancer Research : BCR·Galatea KallergiVassilis Georgoulias

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Citations

Nov 15, 2020·Journal of Experimental & Clinical Cancer Research : CR·Cristina Alexandra Ciocan-CartitaCornelia Braicu
Feb 27, 2021·Computational Biology and Chemistry·Feng LiZhiyu Wang
Apr 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Liu YangWei Wei
Jul 31, 2021·Expert Review of Molecular Diagnostics·Evagelia ChantzaraAthanasios Kotsakis

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Datasets Mentioned

BETA
GSE19783
GSE31364
GSE9574
GSE18672
GSE27562
GSE16443
GSE15852
GSE12763

Methods Mentioned

BETA
xenograft

Software Mentioned

Bioconductor
siggenes
Significance Analysis of Microarrays ” ( SAM )
R
Pathway Commons PC
SPSS
ARIOL system
ARIOL
WebGestalt
WebGestalt ( WEB - based GEne SeT AnaLysis Toolkit )

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