The Prognostic Value of Pyrosequencing-Detected MGMT Promoter Hypermethylation in Newly Diagnosed Patients with Glioblastoma

Disease Markers
Veronica VillaniMariantonia Carosi

Abstract

O6-methylguanine-DNA-methyltransferase (MGMT) has emerged as a relevant predictor of therapeutic response and good prognosis in patients with glioblastoma (GBM). Transcriptionally active MGMT rapidly removes the alkyl adducts, preventing the formation of cross-links and thereby causing resistance to alkylating drugs. Studies with pyrosequencing (PSQ) showed that this technique has a higher reproducibility and sensitivity than other techniques. However, the definition of a prognostically relevant threshold for the percentage of MGMT methylation remains one of the most critical issues in the use of PSQ analysis. The aim of this study was to define the cut-off value correlated with good favourable prognostic outcomes. We retrospectively analyzed 51 patients (33 males, 18 females) with GBM who underwent surgery or biopsy. The Receiver Operating Characteristics analysis showed that the best possible criteria for PSQ-detected percentage of MGMT methylation that predicted progression-free survival (PFS) and overall survival (OS) were 19% and 13%, respectively. Patients with ≤ 19% of PSQ-detected MGMT had a shorter PFS (HR: 0.24, p < 0.01); those ones with ≤ 13% had a shorter OS (HR: 0.33, p < 0.05). Our study reinforces the importance...Continue Reading

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Citations

Feb 6, 2017·Clinical Epigenetics·Esperanza Martín-SánchezDavid Guerrero-Setas
Feb 9, 2017·Oncotarget·Esperanza Martín-SánchezDavid Guerrero-Setas
May 7, 2019·Journal of Neuropathology and Experimental Neurology·Rikke H DahlrotBjarne Winther Kristensen

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Methods Mentioned

BETA
surgical resection
biopsy
PCR

Software Mentioned

Statistical Package for Social Sciences
PSQ
PyroMarker CpG

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