The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis

PLoS Biology
Patrick EichenbergerRichard Losick

Abstract

Asymmetric division during sporulation by Bacillus subtilis generates a mother cell that undergoes a 5-h program of differentiation. The program is governed by a hierarchical cascade consisting of the transcription factors: sigma(E), sigma(K), GerE, GerR, and SpoIIID. The program consists of the activation and repression of 383 genes. The sigma(E) factor turns on 262 genes, including those for GerR and SpoIIID. These DNA-binding proteins downregulate almost half of the genes in the sigma(E) regulon. In addition, SpoIIID turns on ten genes, including genes involved in the appearance of sigma(K). Next, sigma(K) activates 75 additional genes, including that for GerE. This DNA-binding protein, in turn, represses half of the genes that had been activated by sigma(K) while switching on a final set of 36 genes. Evidence is presented that repression and activation contribute to proper morphogenesis. The program of gene expression is driven forward by its hierarchical organization and by the repressive effects of the DNA-binding proteins. The logic of the program is that of a linked series of feed-forward loops, which generate successive pulses of gene transcription. Similar regulatory circuits could be a common feature of other systems...Continue Reading

Associated Datasets

Jun 6, 2010·Shane T JensenPatrick Yves Eichenberger

References

Mar 11, 1992·Journal of Bacteriology·S W SatolaC P Moran
Apr 20, 1990·Journal of Molecular Biology·L B Zheng, R Losick
Mar 1, 1990·International Journal of Food Microbiology·K Shinagawa
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S LuL Kroos
Nov 1, 1990·Journal of General Microbiology·A Kuroda, J Sekiguchi
Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Feb 1, 1989·Genes & Development·C Karmazyn-CampelliP Stragier
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T J KenneyC P Moran
Jul 5, 1987·Journal of Molecular Biology·W DonovanR Losick
Feb 1, 1986·Journal of General Microbiology·I Lopez-DiazJ Mandelstam
Jan 1, 1986·Annual Review of Genetics·R LosickP J Piggot
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T L LaBellW G Haldenwang
Nov 1, 1986·Journal of General Microbiology·S Cutting, J Mandelstam
Oct 13, 1995·Journal of Molecular Biology·K M TattiC P Moran
Feb 1, 1995·Journal of Bacteriology·N Frandsen, P Stragier
Feb 15, 1995·Genes & Development·J A Londoño-Vallejo, P Stragier
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L KarowP J Piggot
Jun 1, 1993·Journal of Bacteriology·K SmithP Youngman

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Citations

Jun 17, 2014·PloS One·Hermann HämmerleUdo Bläsi
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Francisco M CamasJuan F Poyatos
Jul 31, 2013·Briefings in Bioinformatics·Hiroyuki KurataTakenori Takata
Sep 11, 2012·Nature Chemical Biology·Germán PlataDennis Vitkup
Nov 18, 2004·Journal of Bacteriology·Jessica M SilvaggiRichard Losick
Feb 3, 2005·Genetics·Edo KussellStanislas Leibler
Oct 4, 2005·Journal of Bacteriology·Keith V Alsaker, Eleftherios T Papoutsakis
Nov 4, 2005·Journal of Bacteriology·Rita ZilhãoAdriano O Henriques
Dec 3, 2005·Journal of Bacteriology·D C McPhersonA Driks
Dec 31, 2005·Journal of Bacteriology·Jessica M SilvaggiRichard Losick
Aug 23, 2006·Journal of Bacteriology·Alisa W SerioAbraham L Sonenshein
Sep 5, 2006·Journal of Bacteriology·Teresa CostaAdriano O Henriques
Sep 26, 2006·Journal of Bacteriology·Farah K TengraAnthony G Garza
Nov 23, 2006·Journal of Bacteriology·Rebecca GiornoAdam Driks
Dec 19, 2006·Journal of Bacteriology·Teresa CostaAdriano O Henriques
Aug 19, 2007·Journal of Bacteriology·Chris Guillot, Charles P Moran
Aug 28, 2007·Applied and Environmental Microbiology·Andreas UrbanChristoph Freiberg
Dec 11, 2007·Journal of Bacteriology·Rachele IsticatoEzio Ricca
Jul 18, 2008·Genome Biology·Shawn W JonesEleftherios T Papoutsakis
Oct 22, 2008·Nature Genetics·Andrew P CapaldiErin K O'Shea
Oct 20, 2009·Journal of Bacteriology·Xiangyun Qiu, Peter Setlow
Feb 27, 2010·PloS One·Yong-Jun Shin, Mehrdad Nourani
May 4, 2010·Journal of Bacteriology·Giuseppina CangianoEzio Ricca
Jul 6, 2010·Journal of Bacteriology·Sanjat KanjilalStephen B Calderwood
Sep 24, 2010·PloS One·Yong-Jun Shin, Leonidas Bleris
Oct 6, 2010·Genome Biology·Peter WaltmanRichard Bonneau
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Meng How TanHarley H McAdams
Dec 3, 2010·PLoS Computational Biology·Christopher Y ParkOlga G Troyanskaya
Mar 3, 2011·Journal of Biochemistry·Daisuke ImamuraKazuhito Watabe
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·David A RaskoJacques Ravel

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Datasets Mentioned

BETA
GSE1620

Methods Mentioned

BETA
footprinting
PCR
immunoprecipitation
atomic force microscopy
in vitro transcription

Software Mentioned

BioProspector
Rosetta Resolver
MDscan
Resolver
BioOptimizer

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