The programmed DNA elimination and formation of micronuclei in germ line cells of the natural hybridogenetic water frog Pelophylax esculentus

Scientific Reports
Magdalena ChmielewskaMaria Ogielska

Abstract

DNA elimination is a radical form of gene silencing and occurs both in somatic and germ cells. The programmed DNA elimination occurs during gametogenesis in interspecies hybrids that reproduce by hybridogenesis (stick insects, fishes, and amphibians) and concerns removal of whole genomes of one of the parental species and production of clonal gametes propagating the genome of the other species. The cellular mechanisms differ considerably in hybridogenetic insects and fishes but remains unknown in edible frogs Pelophylax esculentus, natural hybrids between Pelophylax lessonae and Pelophylax ridibundus. Here we report DNA elimination mechanism in early developing gonads of diploid and triploid hybrid frogs, studied by TEM, immunofluorescence, and cytochemistry. In gonocytes of both sexes (primary oogonia and prespermatogonia), micronuclei emerge as detached nuclear buds formed during interphase. We found depletion of nuclear pore complexes in micronuclear membrane and chromatin inactivation via heterochromatinization followed by degradation of micronuclei by autophagy. Micronuclei formation does not lead to apoptotic cell death showing that genome elimination is a physiological process. Chromatin elimination via micronuclei in P....Continue Reading

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Citations

Feb 22, 2021·Current Opinion in Cell Biology·Ksenia KrupinaDon W Cleveland
Feb 17, 2021·Genome Biology and Evolution·Zuzana MajtánováTariq Ezaz
May 9, 2021·Genome Biology and Evolution·Alexander Suh, Anne-Marie Dion-Côté
Jul 18, 2020·Current Biology : CB·Jianbin WangRichard E Davis
Sep 21, 2021·Biological Reviews of the Cambridge Philosophical Society·Dmitrij Dedukh, Alla Krasikova

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Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy
PCR
Assay

Software Mentioned

Fiji
STATISTICA
KS400
GENEMAPPER
Imaris
Adobe Photoshop
Leica LasAF

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