The proinflammatory actions of angiotensin II are dependent on p65 phosphorylation by the IkappaB kinase complex.

The Journal of Biological Chemistry
Annie DouilletteMarc J Servant

Abstract

The vasoactive hormone angiotensin II (Ang II) probably triggers inflammatory cardiovascular diseases by activating transcription factors such as NF-kappaB. We describe here a novel mode of NF-kappaB activation in cultured vascular smooth muscle cells exposed to Ang II. Ang II treatment resulted in an increase in the phosphotransferase activity of the IKK complex, which was mediated through the AT1 receptor subtype. The typical phosphorylation and proteasome-dependent degradation of the NF-kappaB inhibitor IkappaBalpha were not observed. Rather, Ang II treatment of vascular smooth muscle cells led to the phosphorylation of p65 on serine 536, a signal detected in both the cytoplasm and the nuclear compartments. The use of pharmacological inhibitors that inhibit the activation of MEK by Ang II revealed that phosphorylation of p65 on serine 536 did not require the MEK-ERK-RSK signaling pathway. On the other hand, specifically targeting the IKKbeta subunit of the IKK complex by overexpression of a dominant negative version of IKKbeta (IKKbeta K44A) or silencing RNA technology demonstrated that the IKKbeta subunit of the IKK complex was responsible for the detected phosphoserine 536 signal in Ang II-treated cells. Characterization o...Continue Reading

References

Apr 18, 1991·The New England Journal of Medicine·M H AldermanJ H Laragh
Mar 1, 1995·Current Opinion in Nephrology and Hypertension·M H Alderman
Aug 26, 1993·The New England Journal of Medicine·T W MeadeW S Peart
Jan 1, 1997·Hypertension·K K GriendlingR W Alexander
Jul 9, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·R KranzhöferW Kübler
Sep 15, 1999·Nature·J A Romashkova, S S Makarov
Oct 16, 1999·The Journal of Biological Chemistry·H SakuraiW Toriumi
Oct 18, 2000·Regulatory Peptides·K M Schmidt-OttM I Phillips
May 25, 2002·Kidney International·Gunter WolfFriedrich Thaiss
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Laure VoisinSylvain Meloche
Aug 13, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Allan R BrasierMohsen S Eledrisi
Apr 5, 2003·The International Journal of Biochemistry & Cell Biology·Yusuke SuzukiJesus Egido
Jul 19, 2003·Circulation Research·Catherine A LemariéStéphanie Lehoux
Dec 11, 2003·Atherosclerosis. Supplements·Renke Maas, Rainer H Böger
Jan 7, 2004·Nature Reviews. Drug Discovery·Michael KarinQ May Wang
Apr 10, 2004·Current Opinion in Nephrology and Hypertension·Valentina Kon, Kathy Jabs
May 4, 2004·Nature Reviews. Molecular Cell Biology·Lin-Feng Chen, Warner C Greene
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ivan MattioliM Lienhard Schmitz
May 8, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Thomas SkurkHans Hauner
May 18, 2004·Trends in Immunology·Giuseppina Bonizzi, Michael Karin
Jun 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·D Scott WitherowRobert J Lefkowitz
Sep 17, 2004·Genes & Development·Matthew S Hayden, Sankar Ghosh

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Citations

Aug 1, 2012·The Journal of Biological Chemistry·Aarthi NarayananFatah Kashanchi
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Marie Cargnello, Philippe P Roux
Feb 21, 2009·Journal of Periodontology·C F SantosM C O Salgado
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ding AiYi Zhu
Dec 18, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Shu LiChenghai Liu
Dec 23, 2011·The Biochemical Journal·Yves RomeoPhilippe P Roux
Aug 25, 2016·Science Signaling·Jean-Philippe PradèreRobert F Schwabe
Jul 31, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Amit Verma, Kapil Mehta
Oct 19, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Priscilla DoyonMarc J Servant
Sep 22, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anna MolesFiona Oakley
Jul 7, 2019·Journal of Experimental & Clinical Cancer Research : CR·Christian Borgo, Maria Ruzzene
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Courtney NicholasAndrea I Doseff
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karin FinkNathalie Grandvaux
Dec 12, 2018·The Journal of Clinical Investigation·Raelene J PickeringMerlin C Thomas
Jul 28, 2019·Journal of Tissue Engineering and Regenerative Medicine·Sahar S Abd El-Rahman, Hany M Fayed
Apr 12, 2013·American Journal of Physiology. Cell Physiology·Thomas A MorinelliMichael E Ullian
Dec 22, 2020·Frontiers in Pharmacology·Zhun XiaoPing Liu
Aug 31, 2021·Oxidative Medicine and Cellular Longevity·Chengyang XuZhipeng Cao

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