The prolonged survival of fibroblasts with forced lipid catabolism in visceral fat following encapsulation in alginate-poly-L-lysine.

Biomaterials
Fangping YangO Ziouzenkova

Abstract

Although alginate-poly-L-lysine (AP(L)) encapsulation of cells producing bioactive peptides has been widely tested, it is unknown whether AP(L) supports lasting catabolic functions of encapsulated cells in adipose tissue, which are required for obesity reduction. We tested functions of AP(L)-encapsulated fibroblasts isolated from wild-type (WT) and aldehyde dehydrogenase 1a1 knockout mice (KO), which resist obesity on a high-fat (HF) diet, have a higher metabolic rate, and express increased levels of thermogenic uncoupling protein-1 (Ucp1) in their deleterious visceral fat depots compared to WT mice. To enable in vivo detection and quantification, fibroblasts were stably transfected with green-fluorescent protein. WT- or KO-containing microcapsules were injected into two visceral depots of WT mice fed an HF diet. Eighty days after transplantation, microcapsules were located in vivo using magnetic resonance imaging. KO microcapsules prevented weight gain in obese WT mice compared to a mock- and WT capsule-injected groups on an HF diet. The weight loss in KO-treated mice corresponded to lipid reduction and induction of thermogenesis in the injected visceral fat. The non-treated subcutaneous fat was not altered. Our data suggest t...Continue Reading

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Citations

Mar 7, 2014·Nutrients·Jennifer M PetrosinoOuliana Ziouzenkova
Jun 13, 2015·Trends in Pharmacological Sciences·Gorka OriveDwaine Emerich
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May 23, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Ziad OmranMohamed Alarjah
Oct 28, 2019·Scientific Reports·Chen GilorOuliana Ziouzenkova
Jul 19, 2017·Xenotransplantation·Kefeng YangOuliana Ziouzenkova

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