The Promising Nanocarrier for Doxorubicin and siRNA Co-delivery by PDMAEMA-based Amphiphilic Nanomicelles

ACS Applied Materials & Interfaces
Qiang ChengZicai Liang

Abstract

Synergistic effects of anticancer drug and siRNA have displayed superior advantages for cancer therapy. Herein, we deeply analyzed the feasibility that whether doxorubicin (DOX) and siRNA could be co-delivered by mPEG-PCL-graft-PDMAEMA (PECD) micelles, which mediated excellent DNA/siRNA delivery in vitro and in vivo reported in our previous work. DOX-loaded NPs (PECD-D) were developed by nanoprecipitation technology and exhibited high drug loading content (DLC, 9.5%). In vitro cytotoxicity study in MDA-MB-231 cells, PECD-D treated groups had lower IC50 compared to free DOX groups (F-DOX) at different transfection time (24, 48, and 72h), which maybe attribute to its high cellular uptake and endosomal escape properties. The speculation was confirmed with the results of drug release profile in acidic media, flow cytometry analysis and confocal images. Futhermore, Cy5 labeled siRNA was introduced in PECD-D micelles (PECD-D/siRNA) to track the behavior of dual-loaded nanodrug in vitro and in vivo. Flow cytometry analysis presented that DOX and siRNA were successfully co-delivered into cells, the positive cells ratio were 94.6 and 99.5%, respectively. Confocal images showed that not only DOX and siRNA existed in cytoplasm, but DOX tr...Continue Reading

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Citations

Jun 9, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ting PengLei Yu
Aug 3, 2016·Biomaterials Science·Erhan KelesYuehe Lin
Mar 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiayi PanVladimir P Torchilin
May 10, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Guangping YuShengsong Tang
Mar 29, 2020·Pharmaceutics·Nataša AvramovićTatjana Simić
Oct 2, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lei LiPeihong Ni
Nov 2, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cao Dai PhungJong Oh Kim
Jan 6, 2021·Nanomaterials·Salah M TawfikYong-Ill Lee
Feb 24, 2020·Biotechnology Advances·Yuhua WengYuanyu Huang
May 7, 2020·Asian Journal of Pharmaceutical Sciences·Yi PengLushan Yu
Dec 27, 2018·International Journal of Pharmaceutics·Shukai DingThierry F Vandamme
Aug 13, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jian SunZhaowei Chen
Jun 23, 2020·Biomacromolecules·Raut BholakantWei Chen

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