The promoter methylomes of monochorionic twin placentas reveal intrauterine growth restriction-specific variations in the methylation patterns

Scientific Reports
Zhiming HeJunwen Wang

Abstract

Intrauterine growth restriction (IUGR) affects the foetus and has a number of pathological consequences throughout life. Recent work has indicated that variations in DNA methylation might cause placental dysfunction, which may be associated with adverse pregnancy complications. Here, we investigated the promoter methylomes of placental shares from seven monochorionic (MC) twins with selective intrauterine growth restriction (sIUGR) using the healthy twin as an ideal control. Our work demonstrated that the IUGR placental shares harboured a distinct DNA hypomethylation pattern and that the methylation variations preferentially occurred in CpG island shores or non-CpG island promoters. The differentially methylated promoters could significantly separate the IUGR placental shares from the healthy ones. Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) further confirmed the genome-wide DNA hypomethylation and the lower level of hydroxymethylation statuses in the IUGR placental shares. The methylation variations of the LRAT and SLC19A1 promoters, which are involved in vitamin A metabolism and folate transportation, respectively, and the EFS promoter were further validated in an additional 12 pairs of MC tw...Continue Reading

References

May 25, 1983·Nucleic Acids Research·M A Gama-SosaM Ehrlich
Jun 1, 1996·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·W SepulvedaK H Nicolaides
Jun 4, 1998·Obstetrics and Gynecology·C V AnanthY L Lai
Jan 28, 1999·The Journal of Biological Chemistry·A RuizD Bok
Sep 3, 2004·American Journal of Obstetrics and Gynecology·Thomas J GariteJames A Thorp
Jun 28, 2005·Nucleic Acids Research·Bing ZhangJay Snoddy
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson
Apr 6, 2006·Bioinformatics·Ryota Suzuki, Hidetoshi Shimodaira
Dec 6, 2006·Nature Reviews. Genetics·Y M Dennis Lo, Rossa W K Chiu
Jun 2, 2007·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·E GratacósJ Deprest
Aug 30, 2008·Journal of Clinical Pathology·B Huppertz
Jan 20, 2009·Nature Genetics·Zachary A KaminskyArt Petronis
Apr 18, 2009·Science·Skirmantas Kriaucionis, Nathaniel Heintz
Jul 21, 2009·American Journal of Reproductive Immunology : AJRI·Matthew A Maccani, Carmen J Marsit
Jul 29, 2009·BMC Bioinformatics·Yuanxin Xi, Wei Li
Jan 15, 2010·The Journal of Nutrition·Parul Christian, Christine P Stewart
Jan 28, 2010·European Journal of Human Genetics : EJHG·Claire Louise Susan TurnerI Karen Temple
May 6, 2010·European Journal of Human Genetics : EJHG·Ryan Kc YuenWendy P Robinson
Oct 21, 2010·Human Reproduction Update·Ewka C M NelissenJohannes L H Evers
Oct 22, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Sonia T ChelbiDaniel Vaiman
May 10, 2011·Nature·William A PastorAnjana Rao
May 18, 2011·Genes & Development·Aimée M Deaton, Adrian Bird
Jun 23, 2011·Reproductive Sciences·Luca LambertiniJia Chen
Jul 16, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Carolyn E BanisterCarmen J Marsit
Nov 30, 2011·Epigenomics·Boris Novakovic, Richard Saffery
Jan 13, 2012·The Journal of Clinical Endocrinology and Metabolism·Steven D Chernausek
Feb 2, 2012·Molecular Medicine Reports·Ourania KoukouraDemetrios A Spandidos
Sep 18, 2012·Cell·Christine Guo LianYujiang G Shi
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lucia L LamMichael S Kobor
Nov 8, 2012·Epigenomics·Sue-Ann Lee, Chunming Ding
May 23, 2013·Physiological Genomics·Pao-Yang ChenMatteo Pellegrini
Jan 18, 2014·Experimental Dermatology·Jessica C HasselAlexandr V Bazhin

❮ Previous
Next ❯

Citations

Jan 21, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Samantha E DayDawn K Coletta
Apr 1, 2017·Nutrition and Cancer·Xiayu WuXu Wang
Mar 3, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Yi ZhangMei Zhong
Jan 4, 2021·Journal of Assisted Reproduction and Genetics·Carla CaniçaisSofia Dória

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE67377

Methods Mentioned

BETA
methylation profiling
PCA
Assay
PCR

Software Mentioned

WebGeStalt
Analyst
MethPrimer
BSMAP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies
Boaz WeiszShlomo Lipitz
Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology
E GratacosJ Deprest
© 2021 Meta ULC. All rights reserved