PMID: 9637069Jun 24, 1998Paper

The promoter of a gene encoding a polygalacturonase-inhibiting protein of Phaseolus vulgaris L. is activated by wounding but not by elicitors or pathogen infection

Planta
Alessandra DevotoG De Lorenzo

Abstract

Polygalacturonase-inhibiting proteins (PGIPs), leucine-rich repeat (LRR) proteins evolutionarily related to several plant resistance genes, bind to and regulate the action of fungal endopolygalacturonases. In Phaseolus vulgaris L., PGIPs are encoded by a gene family comprising at least five members. As a start for a systematic analysis of the regulation of the pgip family, we have analysed the ability of the promoter of the bean gene pgip-1 to direct expression of beta-glucuronidase (GUS) in transfected tobacco protoplasts, microbombarded bean and tobacco leaves, and transgenic tobacco plants. In protoplasts, the pgip-1 gene region from nucleotide (nt) -2004 to nt +27 directed a level of expression that was as high as that directed by the cauliflower mosaic virus (CaMV) 35S promoter and could not be further induced by elicitor treatment; alteration of the region immediately following the TATAA sequence at nt -29 abolished expression. Upon stable integration into tobacco plants of the pgip-1 promoter-GUS construct, as well as of a -394 deletion, expression was detected for both constructs mainly in the stigma and, to a lesser extent, in the anthers and in the conductive vascular tissue. The promoter responded to wounding but not...Continue Reading

Citations

Jun 10, 2000·The Plant Journal : for Cell and Molecular Biology·D AlexM L Chye
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Nov 11, 2008·Biochemistry. Biokhimii︠a︡·M A ProtsenkoN P Korableva
Nov 10, 2001·Annual Review of Phytopathology·G De LorenzoF Cervone
Sep 7, 2000·Molecular Plant-microbe Interactions : MPMI·A L PowellJ M Labavitch
Jun 6, 2009·Seminars in Cell & Developmental Biology·Stijn LagaertGuido Volckaert
Jan 13, 2006·Trends in Plant Science·Luca FedericiFelice Cervone
Jul 1, 2006·Critical Reviews in Microbiology·Cuixia DiLizhe An

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