The promoters of two isoflavone synthase genes respond differentially to nodulation and defense signals in transgenic soybean roots

Plant Molecular Biology
Senthil SubramanianOliver Yu

Abstract

Isoflavonoids are a group of secondary metabolites common to leguminous plants that play roles in nodulation and defense responses. Isoflavone synthase (IFS) catalyzes the key entry point step of isoflavone biosynthesis from the general phenylpropanoid pathway. We have cloned the 5' upstream regions of the genes encoding the two isoflavone synthase isoforms from soybean. We characterized the tissue-specific expression patterns of IFS1 and IFS2 genes of soybean by quantitative RT-PCR. Isoflavone synthase transcripts were detected primarily in the roots and seeds. In transgenic soybean plants in which the IFS1 promoter drove expression of the bacterial uidA (GUS) gene, the expression was localized to the root epidermis and root hairs. We detected differential tissue-specific expression of IFS1:GUS in response to nodulation and defense signals. Bradyrhizobium japonicum, a diazotropic symbiont of soybean, induced the expression of IFS1:GUS in root hairs and xylem poles in the young differentiation zone of the root. This observation suggests novel roles for isoflavonoids in nodulation. The defense related hormone salicylic acid induced the expression of IFS1:GUS in all cell types of the root. We also noticed corresponding alteration...Continue Reading

Citations

Jul 25, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Juan Jose Gutierrez-GonzalezDavid A Sleper
Mar 24, 2010·Applied Microbiology and Biotechnology·Hai DuYixiong Tang
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Apr 15, 2011·Journal of Visualized Experiments : JoVE·Ying DengOliver Yu
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