The promotion of angiogenesis induced by three-dimensional porous beta-tricalcium phosphate scaffold with different interconnection sizes via activation of PI3K/Akt pathways

Scientific Reports
Xin XiaoZhen Wang

Abstract

The porous architectural characteristics of biomaterials play an important role in scaffold revascularization. However, no consensus exists regarding optimal interconnection sizes for vascularization and its scaffold bioperformance with different interconnection sizes. Therefore, a series of disk-type beta-tricalcium phosphates with the same pore sizes and variable interconnections were produced to evaluate how the interconnection size influenced biomaterial vascularization in vitro and in vivo. We incubated human umbilical vein endothelial cells on scaffolds with interconnections of various sizes. Results showed that scaffolds with a 150 μm interconnection size ameliorated endothelial cell function evidenced by promoting cell adhesion and migration, increasing cell proliferation and enhancing expression of platelet-endothelial cell adhesion molecules and vascular endothelial growth factor. In vivo study was performed on rabbit implanted with scaffolds into the bone defect on femoral condyles. Implantation with scaffolds with 150 μm interconnection size significantly improved neovascularization as shown by micro-CT as compared to scaffolds with 100 and 120 μm interconnection sizes. Moreover, the aforementioned positive effects ...Continue Reading

References

Apr 1, 1993·Biomaterials·G CiapettiA Pizzoferrato
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Jul 13, 2002·Journal of Biomedical Materials Research·Jianxi LuJean-Pierre Proust
Jul 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·John StreetEllen H Filvaroff
Nov 19, 2002·Developmental Biology·Alexander M Simon, Andrea R McWhorter
Nov 26, 2002·Cardiovascular Research·Józef Dulak, Alicja Józkowicz
Dec 9, 2003·Journal of Biomedical Materials Research. Part a·Daniel DrueckeHeinz Herbert Homann
Mar 5, 2004·Journal of Biomedical Materials Research. Part a·Sarah CartmellRobert Guldberg
Mar 11, 2004·Tissue Engineering·Molly K SmithDavid J Mooney
Jun 18, 2004·American Journal of Physiology. Cell Physiology·Christopher D O'BrienHorace M DeLisser
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·J X LuB Thierry
Sep 18, 2004·Journal of Biomedical Materials Research. Part a·Michael J MooreMichael J Yaszemski
Jan 22, 2005·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Lia RimondiniRoberto Giardino
Apr 30, 2005·Biomaterials·Vassilis Karageorgiou, David Kaplan
Jun 21, 2005·Nature Biotechnology·Shulamit LevenbergRobert Langer
Apr 13, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D DallariA Giunti
Aug 10, 2006·Journal of Materials Science. Materials in Medicine·S KochG C M Steffens
Jun 11, 2008·Biomaterials·Neil DaviesPeter Zilla
Jul 1, 2008·Trends in Biotechnology·Jeroen RouwkemaClemens A van Blitterswijk
Apr 21, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Patricia A Soucy, Lewis H Romer

❮ Previous
Next ❯

Citations

Mar 28, 2016·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Olli-Pekka LappalainenGeorge K B Sándor
Aug 3, 2016·Trends in Biotechnology·Angad Malhotra, Pamela Habibovic
Sep 7, 2016·Regenerative Medicine·Alessandra BielliAugusto Orlandi
Nov 4, 2016·Journal of Biomedical Materials Research. Part a·Nathaniel J HogrebeKeith J Gooch
Jun 22, 2016·PloS One·Daniel J ModulevskyAndrew E Pelling
Jul 22, 2016·Critical Reviews in Biotechnology·Florian S FruehMatthias W Laschke
May 31, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Daniel Crístian Ferreira SoaresArmando Silva-Cunha
Oct 28, 2017·Advanced Healthcare Materials·Darlin LantiguaGulden Camci-Unal
Nov 4, 2017·Journal of Drug Targeting·Ana Luiza Chaves MaiaDaniel Crístian Ferreira Soares
Nov 25, 2017·Advanced Healthcare Materials·Hwan D KimNathaniel S Hwang
Jan 23, 2018·Bone Research·Hockin Hk XuMark A Reynolds
Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Mohammad Ali NilforoushzadehMehdi Nouradini
Jan 5, 2020·Biomechanics and Modeling in Mechanobiology·Ziying ZhangJingda Ni
Apr 4, 2017·Orthopaedic Surgery·UNKNOWN Microsurgery Department of the Orthopedics Branch of the Chinese Medical Doctor AssociationUNKNOWN Microsurgery and Reconstructive Surgery Group of the Orthopedics Branch of the Chinese Medical Association
Apr 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Huifeng ShaoZhongru Gou
Mar 3, 2017·Journal of Materials Science. Materials in Medicine·Wei ZhuXisheng Weng
Apr 27, 2018·Journal of the Royal Society, Interface·Mathew BaldwinAndrew Carr
Mar 3, 2020·Biomedical Materials·Mahboubeh JafarkhaniMohammad Ali Shokrgozar
Jun 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Werner E G MüllerXiaohong Wang
May 28, 2019·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yunpeng BaiYumi Matsuzaki
Aug 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yonggang Lv, Chongwen Lin
Dec 8, 2015·Journal of Biomaterials Applications·T GenovaF Mussano
Oct 9, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·S SwethaN Selvamurugan
Aug 20, 2019·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Akira MurayamaKatsushi Takeshita

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
enzyme-linked immunosorbent assay
protein assay
X-ray

Software Mentioned

Microview ABA
SPSS

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.