The promyelinating properties of androstenediol in gliotoxin-induced demyelination in rat corpus callosum

Neuropathology and Applied Neurobiology
Samah Kalakh, Abdeslam Mouihate

Abstract

Experimental evidence has shown that the adrenal steroid hormone, androstenediol, dampens the symptoms of demyelination. However, the cellular and molecular effects of androstenediol are not yet known. In the present study, we investigated the cellular and subcellular effects of this hormone in a gliotoxin-induced demyelination model. Male Sprague Dawley rats received 2 μl of either saline or the gliotoxin ethidium bromide (EB, 0.04%) into the corpus callosum. These rats received daily subcutaneous injections of either oil or androstenediol (5 mg/kg). Their brains were collected at 2, 7, 14 and 28 days post-EB injection. Demyelinated lesions were assessed using Luxol fast blue staining. Immunofluorescent staining was used to investigate the number of oligodendrocyte progenitor cells, their maturation and microglial activation at the lesion site. Remyelination was further explored using transmission electron microscopy. The expression levels of total and phosphorylated MBP isoforms and CNPase were explored using western blot. Androstenediol decreased the size of demyelinated lesions in the corpus callosum at 7 and 14 days post-EB injection. It enhanced the number of oligodendrocyte precursor cells, promoted an increase in the nu...Continue Reading

References

Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C DuS Sriram
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maurizio BifulcoJ Wolff
Feb 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Abdeslam MouihateQuentin J Pittman
Feb 16, 2005·Developmental Neuroscience·Mireya Marin-HusstegePatrizia Casaccia-Bonnefil
Jan 18, 2006·Clinical Neurology and Neurosurgery·Sandra Vukusic, Christian Confavreux
Jan 18, 2006·Brain : a Journal of Neurology·Christian Confavreux, Sandra Vukusic
Jun 24, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Laurie Beth J MoralesRhonda R Voskuhl
Aug 12, 2006·Multiple Sclerosis : Clinical and Laboratory Research·Nieves TéllezXavier Montalban
Feb 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher GreggSamuel Weiss
May 29, 2007·International Review of Neurobiology·Robert N Schwendimann, Nadejda Alekseeva
Aug 8, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Peter H JellinckKaren Bulloch
Sep 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Seema Tiwari-WoodruffRhonda R Voskuhl
Apr 9, 2008·Journal of Neurology·Robin J M Franklin, Mark R Kotter
Sep 19, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jean E Merrill
Feb 19, 2009·Journal of Neuroscience Research·Bettina GerstnerPaul A Rosenberg
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Arnaud Nicot
May 27, 2009·American Journal of Epidemiology·Ross L PrenticeJacques E Rossouw
Jun 2, 2009·Journal of Neuroscience Methods·Daniel K CrawfordSeema K Tiwari-Woodruff
Feb 10, 2010·The Journal of Cell Biology·S Y Christin Chong, Jonah R Chan
Sep 23, 2010·Brain : a Journal of Neurology·Daniel K CrawfordSeema K Tiwari-Woodruff
Jan 1, 2009·Therapeutic Advances in Neurological Disorders·Ida S HaussleiterGeorg Juckel
Jan 21, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hartmut B F PohlUeli Suter
Mar 5, 2011·British Journal of Pharmacology·Cris S ConstantinescuBruno Gran
Mar 23, 2011·Journal of Cellular and Molecular Medicine·Shuhui ZhengTinghuai Wang
Nov 11, 2011·Glia·Marta OlahHendrikus W G M Boddeke
Feb 1, 2012·Frontiers in Neuroendocrinology·Markus KippCordian Beyer
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Feb 13, 2013·Journal of Neurochemistry·George Harauz, Joan M Boggs
Feb 19, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Jiong ChenSheng-Xiang Lin
Jul 9, 2013·Journal of Comparative Pathology·A H CrawfordR J M Franklin
Jul 19, 2013·Biochemical Society Transactions·Camilla Norrmén, Ueli Suter
Jul 23, 2013·Nature Neuroscience·Veronique E MironCharles Ffrench-Constant
Nov 1, 2013·Clinical Pharmacology and Therapeutics·O H KantarciM Rodriguez
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anna J KhalajSeema Kaushalya Tiwari-Woodruff
Dec 18, 2013·Immunology·Sandra AmorJohannes M van Noort
Jan 22, 2014·Glia·Robin J M Franklin, Vittorio Gallo

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Citations

Jul 15, 2015·Clinical Neurology and Neurosurgery·Yuan WuXing Wei
Sep 24, 2016·Molecular Neurobiology·Samah Kalakh, Abdeslam Mouihate
May 24, 2019·Scientific Reports·Samah Kalakh, Abdeslam Mouihate
Mar 11, 2017·Frontiers in Cellular Neuroscience·Samah Kalakh, Abdeslam Mouihate
Jul 19, 2017·CNS Neuroscience & Therapeutics·Abdeslam MouihateSamah Kalakh
May 14, 2020·International Immunopharmacology·Mengli CuiBei Zhang
Nov 4, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sik Yu So, Tor C Savidge

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