The pronase resistance of cholesterol-nucleating glycoproteins in human bile

Gastroenterology
A I ZijlstraA K Groen

Abstract

Many putative pronucleating proteins have been isolated from the biliary concanavalin A (con A)-binding fraction. The pronase resistance of the overall nucleating-promoting activity was almost never taken into consideration. The aim of this study was to identify the major pronase-resistant con A-binding glycoproteins. Pronase-treated and -untreated con A-binding glycoproteins were separated on a Superose 12 gel permeation column (Pharmacia, Uppsala, Sweden) and tested in a crystal growth assay. Proteins were identified by amino-terminal sequencing. Con A-binding pronucleating activity eluted in two peaks on the Superose column. This activity was unaltered after pronase treatment. Activity peak I contained too little protein to allow amino-terminal sequencing. In activity peak II, the major pronase-resistant con A-binding glycoproteins were identified as alpha 1-antitrypsin and alpha 1-antichymotrypsin. The 130-kilodalton nucleation promoter was identified as aminopeptidase N, but the full pronase resistance of this protein, reported earlier, was not confirmed. Immunoabsorptive removal of alpha 1-antitrypsin and alpha 1-antichymotrypsin and immunopurification showed that only alpha 1-antichymotrypsin had pronucleating activity. ...Continue Reading

Citations

Nov 8, 2003·World Journal of Gastroenterology : WJG·Jian-Bin XiangYan-Ling Zhang
Dec 15, 2000·Antiviral Research·S BaronJ Pan
May 16, 2003·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·P PortincasaG Palasciano
Apr 13, 1999·Gastroenterology Clinics of North America·C W Ko, S P Lee
Aug 3, 2001·Primary Care·A N Kalloo, S V Kantsevoy

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