PMID: 4897941Sep 1, 1969Paper

The properties of a nuclear acidic protein fraction that binds [6,7-3H]oestradiol-17beta

The Biochemical Journal
R J KingA W Steggles

Abstract

1. Additional evidence was obtained that the nuclear oestradiol-17beta receptor is an acidic protein. Partial purification of the receptor protein was obtained by chromatography on hydroxyapatite and it contains protein-bound phosphate. 2. The nuclear ;5s' and cytoplasmic ;9.5s' and ;5s' receptors from uterus, dimethylbenzanthracene-induced mammary adenocarcinoma and kidney are precipitated together with bound oestradiol-17beta by protamine sulphate. This common property suggests that the nuclear and cytoplasmic receptors are related to each other. 3. The properties of two acidic protein fractions from both liver and dimethylbenzanthracene-induced mammary adenocarcinoma are described. Fraction 1 contains two major components and fraction 2 contains one component, as judged from polyacrylamide-gel electrophoresis. Fraction 2 contains RNA and both fractions contain protein-bound phosphate. 4. These fractions form insoluble complexes with calf thymus histone, protamine sulphate and poly-l-lysine. The formation of these complexes is markedly affected by ionic strength and pH. Ionization of both the in-amino group of lysine and carboxyl group are involved. RNA and DNA do not appear to be involved. The interaction is not affected by ...Continue Reading

Citations

Jan 1, 1980·Histochemistry·M DavidoffT H Schiebler
Mar 11, 1977·Annals of the New York Academy of Sciences·T C SpelsbergD Wells
Dec 6, 1972·Nature: New Biology·R J King, J Gordon
Jun 24, 1976·The New England Journal of Medicine·L Chan, B W O'Malley
Sep 1, 1977·Journal of Toxicology and Environmental Health·T C SpelsbergG Pikler
Jun 1, 1971·The Journal of Urology·P C Walsh, S G Korenman
Jan 1, 1979·Pharmacology & Therapeutics·S J Segal, S S Koide
Jun 22, 1972·Biochimica Et Biophysica Acta·A C NotidesJ H Rudolph
Dec 19, 1979·Biochimica Et Biophysica Acta·G Leclercq, J C Heuson
Jul 1, 1975·Journal of Steroid Biochemistry·A EideS Kvinnsland
Mar 1, 1975·Journal of Steroid Biochemistry·I S Edelman
Jan 1, 1973·Journal of Steroid Biochemistry·P JouanM L Thieulant
Nov 1, 1976·Journal of Steroid Biochemistry·T C SpelsbergD Wells
Sep 1, 1973·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G Leclercq, J C Heuson
Jun 1, 1972·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A W Steggles, R J King
Oct 1, 1984·Analytical Biochemistry·M S DenisonC F Wilkinson
Dec 1, 1976·Current Problems in Cancer·G Leclercq, J C Heuson
Mar 1, 1974·Science·G S SteinL J Kleinsmith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.