The Propionibacterium freudenreichii hemYHBXRL gene cluster, which encodes enzymes and a regulator involved in the biosynthetic pathway from glutamate to protoheme

Applied Microbiology and Biotechnology
Y HashimotoY Murooka

Abstract

A clone that can complement both Escherichia coli hemB and hemL mutations was found among plasmids containing the Propionibacterium freudenreichii hemB gene, which encodes delta-aminolevulinic acid dehydratase. The regions upstream and downstream of the hemB gene were sequenced. Two open-reading frames (ORF1 and -2), which were similar to the hemY gene encoding protoporphyrinogen oxidase and the hemH gene encoding ferrochelatase from Bacillus subtilis, were found upstream of the hemB gene. ORF1 and -2 complemented the E. coli hemG mutation, defective in protoporphyrinogen oxidase, and the hemH gene respectively. Since ORF1 had no homology to hemG, the gene was designated hemY. The hemYHB genes appeared to be within the same transcription unit. Downstream from the hemB gene, three open-reading frames were found. One of these, transcribed in the same direction as the hemB gene, was identical to be the hemL gene, which encodes glutamate-1-semialdehyde 2,1-aminomutase. The other two open-reading frames, located between the hemYHB and hemL genes, were transcribed divergently, and their deduced amino acid sequences showed similarities to a membrane-bound transport protein and a transcriptional regulatory protein respectively. The two...Continue Reading

Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·Pornpimon Kiatpapan, Yoshikatsu Murooka
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yongzhe PiaoYoshikatsu Murooka
Dec 3, 2004·Applied and Environmental Microbiology·Yongzhe PiaoYoshikatsu Murooka
Dec 8, 2005·Applied and Environmental Microbiology·Dag Anders BredeHelge Holo
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Aug 12, 2005·Seminars in Cutaneous Medicine and Surgery·Holger Brüggemann
Sep 4, 2001·Biotechnology & Genetic Engineering Reviews·S Nishikawa, Y Murooka
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Leslie Cuthbertson, Justin R Nodwell
Oct 24, 1998·Archives of Biochemistry and Biophysics·A V CorrigallP N Meissner

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