The prostacyclin analogue carbacyclin inhibits Ca(2+)-activated K+ current in aortic baroreceptor neurones of rats

The Journal of Physiology
Z LiF M Abboud

Abstract

1. Previous studies indicate that prostacyclin (PGI2) increases the activity of baroreceptor afferent fibres. The purpose of this study was to test the hypothesis that PGI2 inhibits Ca(2+)-activated K+ current (IK(Ca))in isolated baroreceptor neurones in culture. 2. Rat aortic baroreceptor neurones in the nodose ganglia were labelled in vivo by applying a fluorescent dye (DiI) to the aortic arch 1-2 weeks before dissociation of the neurones. Outward K+ currents in baroreceptor neurones evoked by depolarizing voltage steps from a holding potential of -40 mV were recorded using the whole-cell patch-clamp technique. 3. Exposure of baroreceptor neurones to the stable PGI2 analogue carbacyclin significantly inhibited the steady-state K+ current in a dose-dependent and reversible manner. The inhibition of K+ current was not caused indirectly by changes in cytosolic Ca2+ concentration. The Ca(2+)-activated K+ channel blocker charybdotoxin (ChTX, 10(-7) M) also inhibited the K+ current. In the presence of ChTX or in the absence of Ca2+, carbacyclin failed to inhibit the residual K+ current. Furthermore, in the presence of high concentrations of carbacyclin, ChTX did not cause further reduction of K+ current. 4. Carbacyclin-induced inhi...Continue Reading

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Citations

Feb 11, 2004·Progress in Biophysics and Molecular Biology·Matthew C Zimmerman, Robin L Davisson
Jan 14, 1998·Brain Research. Molecular Brain Research·S S MeyrellesM W Chapleau
Dec 21, 2002·Neuroscience Letters·Kenneth Lamb, Klaus Bielefeldt
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Feb 18, 2011·American Journal of Physiology. Cell Physiology·Bai-Yan LiJohn H Schild
May 5, 2007·The Journal of Physiology·Vladislav SnitsarevFrançois M Abboud
Aug 3, 2005·Hypertension·Vladislav SnitsarevFrancois M Abboud
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Oct 23, 2009·American Journal of Physiology. Cell Physiology·Libin ZhangYu-Long Li
Oct 6, 2016·Journal of Computational Neuroscience·Jacob SturdyMette S Olufsen
Jul 19, 2001·Annals of the New York Academy of Sciences·M W ChapleauF M Abboud
Mar 18, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Choong-Ku LeeSeong-Woo Jeong
Mar 10, 2000·American Journal of Physiology. Heart and Circulatory Physiology·Z L Guo, J C Longhurst

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